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20 Phase I Selections from the 98.1 Solicitation

(In Topic Number Order)
AGAVE BIOSYSTEMS, INC.
117 Devlen Rd
Groton, NY 13073
Phone:
PI:
Topic#:
(607) 255-2890
Dr. Carl A. Batt
CBD 98-101
Title:Labeless, Reagentless, Biosensor
Abstract:The preponderance of biosensors under development today rely on labeling reagents such as fluorescent, radioisotopic or enzymatic tags. As a result, the added complexity of these reagents and their incorporation into the detection system has resulted in designs that are difficult to implement or that require significant sample preparation steps before introduction into the detection instrument. While increasing signal, these labels also increase noise, can negatively impact on specificity and overall in the signal-to-noise ratio. In short, the need for reagents by current systems has created significant obstacles to fielding a truly portable, reliable and easy to use biosensor, i.e., one that can be used by the warfighter without significant training or preparation. To meet these needs, Agave BioSystems proposes to develop a truly labeless and reagentless biosensor based upon the optical diffraction of analyte bound to reflective silicon. Key to this effort is the innovative, proprietary microcontact printing technology of Agave BioSystems and its collaborator. This technology allows the precise placement of arrays of biological recognition molecules to form gratings which coupled with optical defraction allows the reagentless detection of multiple targets.

PHYSICAL OPTICS CORP.
2520 W. 237th Street, R & D Division
Torrance, CA 90505
Phone:
PI:
Topic#:
(310) 530-7130
Lothar U. Kempen, Ph.D.
CBD 98-101
Title:High Resolution Imaging Ellipsometer for Labeless, Multi-Channel Immunosensing
Abstract:We propose to develop a real-time imaging optical arrangement monitoring a sample solution for a large number of analytes in parallel. Using a photo-induced immobilization technique, a multitude of different antibodies is immobilized on the surface of a glass carrier, each type located at a specific position on the chip. When sample fluid containing the analytes lows over the sensor surface, the top plane of the optical carrier chip is interrogated from below by an imaging ellipsometer to provide a spatially resolved measurement of the sample surface in approximately one second. This allows precise monitoring of the film thickness variations due to a binding event with monolayer resolution. The use of several spectral bands for performing the measurement adds another parameter in addition to film thickness determination to enhance the sensitivity. The imaging will be performed with about 10 micrometer lateral resolution, leading to highly resolved fast information about the binding status of a vast number of different antibodies without the need for labeling reagents. The optical setup can also be used with minor modifications to monitor DNA binding events. This system will be of high commercial interest for chemical and biological screening and for environmental monitoring applications.

AMERICAN RESEARCH CORP. OF VA
1509 Fourth St
Radford, VA 24143
Phone:
PI:
Topic#:
(540) 731-0655
Dr. Russell J. Churchill
CBD 98-102
Title:Minature Biological Detector Using On-Chip Eletrostatic Spray Separation
Abstract:Recent developments in the world political arena have led to The need for miniaturized instrumentation for rapid characterization of micro-organisms and toxins to protect military and civilian personnel against biological weapons and to allow timely administration of prophylactics. Considerable effort is currently underway to develop polymerase chain reaction (PCR) assays with automated sample preparation. Although centrifugation and immunomagnetic particle methods of sample preparation have been evaluated, the need for sample separation prior to PCR on unattended biological detectors remains unanswered. This proposal suggests the use of electrostatic spray injection as a means of sample preparation prior to PCR methods of analysis. The innovation of the proposed sensor system is the combination of charge-based and mass-based separation operations within a micromachined flow manifold to provide a detector having unparalleled sensitivity to micro-organisms and toxins. The Phase I Technical Objectives include design and fabrication of a laboratory electrostatic spray system, evaluation of the efficiency of electrostatic particle separation and acquisition of families of test data for use in optimization of a proof-of-concept prototype system. In Phase II, test procedures will be automated and the sensor will be miniaturized for use at an Army facility.

GALA DESIGN LLC
Po Box 520
Sauk City, WI 53583
Phone:
PI:
Topic#:
(608) 643-0152
Robert D. Bremel
CBD 98-103
Title:Large Scale Production of Antibodies in Transgenic Animals
Abstract:We propose a rapid and easily scaleable system for the production of target quantities of monoclonal antibodies in the milk of cattle. O ur approach utilizes a modification of gene therapy techniques to di rectly insert genes for the monoclonals of interest into the mammary epithelium of cattle. In Phase I, we will develop appropriate vector constructs and demonstrate their fidelity in vitro, in preparation f or a rapid transition to production.

F&S, INC.
P.O. Box 11704
Blacksburg, VA 24062
Phone:
PI:
Topic#:
(540) 953-4267
Scott Meller
CBD 98-104
Title:Novel Microcantilever Biosensor for the Measurement of Biomolecular Forces
Abstract:The ability to detect minute quantities of toxic biological substance s will provide the ability to quickly assess a situation so that an a ppropriate response to exposure can be orchestrated. Not only will t he development of this technology be important in toxic agent warfare detection, the biological sensors would be pertinent in commercial ap plications such as process control and point-of-care diagnostics. Be cause rapid diagnosis of medical situations can result in better pat ient care, there is a great desire to have portable test facilities, bases on affinity sensing technology, that can produce analysis insta ntaneously. The main advantage of affinity sensors is that separatio n procedures are not reqired thereby providing results with specific binding of select target molecules. Devices can be small, rugged, and can demonstrate sensitivity levels equal to or greater than tradi tional instrumentation. Interest in these devices has grown steadily with the recent advent of inexpensive, mass-produced MEMS devices. M ore specifically, microcantilevers can now be produced to detect the presence of biological samples through changes in resonance frequenc y, deflection, amplitude, and Q-factor. F&S proposes to commercializ e a microcantilever sensor that is capable of measuring.

SYSTEMS & PROCESSES ENGINEERING CORP.
P.O. Box 162487
Austin, TX 78716
Phone:
PI:
Topic#:
(512) 306-1100
Mike Durrett, Ph.D.
CBD 98-104
Title:Biological Threat Identification Employing Force Amplified Biosensor Technology
Abstract:Systems & Processes Engineering Corporation (SPEC) proposes to develop a highly sensitive point pathogen detection system based on Force Amplified Biosensor (FAB) technology. Similar to Atomic Force Microscopy (AFM). FAB employs a greatly simplified AFM configuration to measure intermolecular interactions. By employing immobilized antibodies and very small, well characterized, magnetic beads, extremely high sensitivity and specificity can be achieved. Further this proposed sensor will expand on the FAB technology by using the force required to break the bonds in the immunoassay as either a discriminant or as an additional selective feature in determining the type of pathogen. The final system will be controlled by a microprocessor and will be designed to be compact and portable. A low volume air sampler will be all that is necessary because of the use of the novel highly sensitive FAB technology coupled with the additional selectivity of bond-force-discrimination.

DIFFRACTION LTD.
P.O. Box 1115
Waitsfield, VT 05673
Phone:
PI:
Topic#:
(802) 496-6642
Scott W. Tighe
CBD 98-106
Title:Advanced Column and Accumulator for Low Power Consumption GC
Abstract:This Phase I research will focus on developing a low power usage chip-based micromachined CC (MMGC) column and sample accumulator. The design of this Innovative miniaturized MMGC will be aimed at reducing the amount of time needed for an analysis. The unique column approach incorporates a stationary phase that will separate organic compounds efficiently. We will investigate the use of several different stationary phase compounds in removable or changeable) MMGC columns or.a multiple column chip. The MMCC chip-based column will be temperature programmable in a multi-zone configuration. Inherent to the design is a unique micromachined organic vapor sampling device employing an innovative microthermal accumulator. Evaluation of this gas chromatographic (GC) column and sample accumulator will be performed using a breadboard set up using organic compounds which are specific in warfare situations.

FEMTOSCAN CORP.
7026 Commerce Park Dr, Suite #2
Midvale, UT 84047
Phone:
PI:
Topic#:
(801) 568-6788
Neil S. Arnold
CBD 98-107
Title:Deve;opment of A Handheld Gas Chromatography/Mass Spectrometry
Abstract:The ability to develop new technology on the basis of existing commercial technology can offer significant cost savings when compared to research programs to develop new technology from scratch. Continuing developments in mass spectrometry (MS), vacuum Systems and high speed gas chromatography (GC) sampling and analysis methods, have created an opportunity to fully demonstrate a powerful handportable GC/MS system in the course of a single Phase I and II SBIR program. This program builds on similar projects at the University of Utah that demonstrated backpack portable GC/MS and high speed roving GC/MS concepts using modified versions of "off-the-shelf" technology. This program will apply new technology in sampling, temperature programmed transfer line GC, low power mass analyzers and lightweight vacuum pumping to create the first handportable GC/MS instrument ready for DOD application testing. This program will be followed by immediate commercialization of the technology for both military and civilian applications.

SYAGEN TECHNOLOGY, INC.
1411 Warner Ave
Tustin, CA 92780
Phone:
PI:
Topic#:
(714) 258-4400
Dr. Jack A. Syage
CBD 98-107
Title:Field-Portable, Real-Time Chemical Analysis Systems
Abstract:Low-cost, high-resolution, field-portable chemical analysis systems are needed to meet critical detection requirements for environmental monitoring and sampling, and for the specific problem of chemical agent battle-field detection and nonproliferation monitoring. New technologies are needed to bring lab-quality performance to the field. An innovative ionization method is proposed that provides for highly specific mass analysis of multicomponent mixtures. The basic principles have been validated and there are no obstacles to transitioning the technology to a compact, field-portable unit that exceeds nearly all performance specifications of the competitive method of gaschromatography, mass spectrometry. The Phase I project will validate the efficiency of detecting chemical weapons convention-scheduled compounds and other broad classes of environmentally important compounds. A variety of sampler options will be examined, and engineering designs for a field-deployable Phase II prototype will be developed.

FOSTER-MILLER, INC.
350 Second Ave
Waltham, MA 02154
Phone:
PI:
Topic#:
(781) 668-4409
William G. Leary
CBD 98-201
Title:CBW Ensemble Pass-Through for A Man Mounted Microclimate Cooling System
Abstract:Current CB protective ensembles impose a thermal burden on aircrew which not only limits mission time. but poses a physiological danger to aircrew operating in even moderate temperature environments~ A small man-mounted cooling system employing active liquid based technology would enhance mission effectiveness and prevent a hazardous rise in core body temperature. However, these state-of-the-art systems were not designed to be used in a CB environment The proposed work will solve this problem through the development of a CB garment pass-through which will allow for the integration of an active cooling system with a CB ensemble. The pass-through may be field installed without the use of special tools, and will not compromise the integrity of the CB garment. Phase I will include the establishment of all relevant design criteria along with a state-of-the-art review of current microclimate cooling systems culminating in the fabrication of a prototype pass-through device. A fully developed CBW ensemble pass-through will be completed in Phase II. Three devices will be produced initially for laboratory analysis in Phase II. Revisions to tile design based on the laboratoryis assessment will be completed, and three full developed prototypes will be manufactured.

FOSTER-MILLER, INC.
350 Second Ave
Waltham, MA 02154
Phone:
PI:
Topic#:
(781) 684-4320
Daniel L. Fischbach
CBD 98-202
Title:CBW Low Profile Man-Mounted Filter And Blower Unit
Abstract:There is an increasing threat of chemical and biological (CB) agents being used by hostile forces when USN aircrew could be exposed. Aircrews must wear special eye and respiratory protective equipment both before. During, and after flight operations, and transition between these states without compromising CB Protectionintegrity. Man-mounted CBW filter/blower units have previously been produced and integrated with the aircrew flight ensemble. However, they are typically bulky and protrude from the front of the aircrew overvest, making it difficult to maneuver the cockpit, and creating a potential snagging hazard for flight controls and during mergency egress. The proposed work addresses these concerns. The unit developed will have a low profile, and be integrated with aircrew's flight ensemble so that the nuisance and safety hazards are eliminated. It will do so while providing the same level of protection as the current units. Phase I will address the individual technologies required to develop the new filter/blower unit; state-of-the-art review, tradeoff analysis, and system design will indicate the best solution. In Phase II, the blower will be manufactured for testing.

MESOSYSTEMS TECHNOLOGY, INC.
MST, 3200 George Washington Way
Richland, WA 99352
Phone:
PI:
Topic#:
(509) 375-3365
Donald J. Hammerstrom
CBD 98-202
Title:An Advanced Gas Mask Incorporating A Low Energy corona Plasma Reactor
Abstract:MesoSystems Technology, Inc. and Battelle Memorial Institute propose to design, fabricate, and evaluate a miniature plasma reactor (MPR) system as an augmentation of the current gas mask filter. A laboratory prototype demonstration (Phase I) will be followed by the development and demonstration of a lightweight, partially ruggedized, fieldable unit (Phase II) which will be delivered to the millitary for further evaluation. The proposed technology effectively treats chemical and biological warfare (CBW) agents simultaneously.

NAVMAR APPLIED SCIENCES CORP.
65 West Street Rd, Suite #B-104
Warminster, PA 18974
Phone:
PI:
Topic#:
(215) 675-4900
Alan Hellman
CBD 98-203
Title:Improved Chemical-Biological (CB) Warfare Ensemble Hood
Abstract:This SBIR proposes the development of a new hood material as an alternative to bromo-butyl rubber for an aircrew B respirator application. This new material provides for a significant improvement for dispersi on of water vapor and heat generated by the head. The new material r etains all the necessary material performance characteristics for Hea d-Eye-Respiratory (HER) protection. The feasibility of bonding this hood material to polycarbonate will be demonstrated. This prototype would represent achievable performance and producibility goals for in corporation into a aircrew respirator hood. Technologies will also b e researched for complimentary approaches to manage head heat transfe r for the combined respirator and flight helmet systems. DOD documen ted data will be used for threat information, aircraft platform IPE r equirements, shipboard operational and maintenance conditions, and CB design/material guidelines. Technical analysis and Phase I evaluatio ns will be accomplished for the new hood material.

FEDERAL FABRICS-FIBERS, INC.
21 Marie Dr
Andover, MA 01810
Phone:
PI:
Topic#:
(978) 470-1859
Zvi Horovitz
CBD 98-204
Title:Light Weight Chemical Protective Hand Wear
Abstract:Current CBW protective flight handwear consists of three pairs of glo ves: an inner cotton liner glove for comfort and perspiration absorpt ion, a 7mil butyl rubber glove for agent protection and a nomex fligh t glove for fire protection. These three pairs of gloves when worn t ogether are very bulky and cumbersome making it very difficult to per form aircrew tasks that require high tactility, such as depressing sm all buttons and switches. The current Nomex glove is particularly bul ky due to the cut-and-sew technique used, which results in extra mate rial on every finger and in every crouch. Federal Fabrics - Fibers (F FF's) will evaluate the current state-of-the-art CB flight glove ense mble technology. Develop and recommend an integrated glove for use b y aircrew and submit material samples. A report shall be delivered t o NAWCADPAX on the recommended design concept. The first task of this project will be to test and evaluate the current CBW three glove syst em. Testing will encompass the areas of comfort and dexterity, chemi cal protection, insulating value and finally fire protective ability. The first task of this project will be to test and evaluate the curre nt CBW three glove system. Testing will encompass the areas of comfo rt and dexterity, chemical protection, insulating value and finally f ire protective ability. The second task is to produce a glove that p rovides equivalent or better protection than the current three glove system with improved comfort and dexterity. FFF will produce, test, a nd deliver both material samples as well as several pairs of gloves. FFF will make our internal test data available to NAWCADPX for compar ison with current system. In addition, FFF will deliver material sam ples and several pairs of gloves to the Navy so they can be evaluated and compared to the current three glove system at

TDA RESEARCH, INC.
12345 W. 52nd Ave
Wheat Ridge, CO 80033
Phone:
PI:
Topic#:
(303) 940-2322
Dr. Bryan M. Smith
CBD 98-205
Title:Water-Based Ensemble Coating
Abstract:Servicemen subject to chemical or biological attack are already supplied with effective defenses, but their external equipment is often not protected from contamination and must often be discarded after contamination. In order to protect the valuable equipment of the aircrew ensemble from exposure to chemical or biological warfare agents, the U.S. Navy has suggested a clear aerosol coating which would facilitate decontamination of the ensemble. TDA Research Inc. has identified a water-based aerosol coating designed to reduce chemical warfare agent residue after a water wash. The clear coating sprays on and is immediately effective in assisting the decontamination of the coated ensemble with water. Once dry, it is not expected to wash off in a simple rain, but can be easily removed under a water stream from a hose. This ensemble coating is expected to also provide the personnel with a measure of additional protection from chemical warfare agents.

TEXAS RESEARCH INSTITUTE AUSTIN, INC.
9063 Bee Caves Rd
Austin, TX 78733
Phone:
PI:
Topic#:
(512) 263-2101
Dr. Michael Dingus
CBD 98-205
Title:CBW Ensemble Protective Clothing
Abstract:Protective clothing technology is a key element in chemical and biological defense, There is a need for a spray-on coating, similar to 3M's Scotch Gard1 which will enhance the barrier properties of standard protective clothing and aid in decontamination. Two approaches have been developed for fabric protection: (1) high surface free energy, low molecular weight resins which are chemically bonded to the fabric and (2) solid film forming polymers which adhere to the fabric. Texas Research Institute Austin Inc. (TRI/Austin) will select and evaluate the best candidates from both classes of barrier resins and develop a coating formulation. The candidates will be tested for permeation, ease of decontamination, ease of application, and fire resistance. TRI/Austin will be assisted by Professor William J. Koros of the University of Texas at Austin, and by TRI/Environmental. TRI/Environmental is the industrial hygiene and environmental testing division of Texas Research International, the parent company of TRI/Austin. TRI/Environmental is an industry-recognized testing lab for protective clothing accredited by the American Industrial Hygiene Association (AIHA) for commercial and government standards. Professor Koros is an expert in barrier polymer research. TRI/Austin has an excellent background in product development for custom polymer formulations, chemical warfare protective equipment, and other applications. The team assembled for this project has the optimum combination of expertise in barrier coatings, polymer formulation, protective clothing testing, and product development.

PHYSICAL SCIENCES, INC.
20 New England Business Center
Andover, MA 01810
Phone:
PI:
Topic#:
(978) 689-0003
Mitchell R. Zakin
CBD 98-206
Title:Polymeric Agent Decontamination System
Abstract:The threat of military and terrorist deployment of chemical weapons has increased alarmingly in recent years. A universal decontamination system is required for safe and effective neutralization of standard and thickened chemical warfare agents (G, V, and H). The system must be non-toxic, non-corrosive, and non-hazardous to equipment and personnel. For effective implementation, the system must be stable, inexpensive, and easy to transport and deploy under field conditions. Current decontamination systems such as DS2 and supertropical bleach are toxic and corrosive. Physical Sciences, Inc. proposes to develop an inexpensive, stable, non-toxic, non-corrosive polymeric decontamination system for G, V, and H agents. The system will be formulated as a water-soluble dry powder to reduce logistical burdens. The system will be effective in either dry or aqueous (reconstituted) form, and will provide a colorimetric indication of the progress of the decontamination reaction. The system will be safe for use on a variety of material including medical decontamination of skin and wounds. In Phase I a prototype system will be formulated and used to demonstrate the rapid, effective decontamination of a material surface coated with standard and thickened agent simulants. A preliminary formulation of the Phase II decontamination system will be completed.

TEXAS RESEARCH INSTITUTE AUSTIN, INC.
9063 Bee Caves Rd
Austin, TX 78733
Phone:
PI:
Topic#:
(512) 263-2101
Dr. Michael Dingus
CBD 98-207
Title:CBW Safe Water Pouch
Abstract:Recent events in the Middle East have focused worldwide attention toward the escalating threat of chemical and biological warfare. Naval Aviators exposed to potential CBW threats need a means to rehydrate themselves in the cockpit. Numerous studies have shown that proper hydration is essential for maximum physical and mental performance. Texas Research Institute Austin, Inc. (TRI/Austin) will team with Trelleborg Viking, Inc. to produce the most CBW resistant water pouch ever devised. A two layer design will result in the best possible agent protection, durability, flexibility, and water potability. Candidate inner bladder materials and threaded penetrator materials will be tested, and the best materials will be selected for the design. The integrity of the mechanical seal between the outer CBW barrier material and the threaded penetrator will be evaluated, along with the integrity of the edge seals in the inner bladder material, using chemical agent simulants as the challenge media. Viral penetration tests and pressure tests will also be performed. The prototype pouch will be further tested against altitude change, temperature, and drop resistance. A draft specification will be prepared that will allow complete replication. A prototype CBW resistant water pouch will be delivered to the Navy.

INVITRO DIAGNOSTICS, INC.
Columbia Univ.Bio Science Park , 3960 Broadway Rm.
New York, NY 10032
Phone:
PI:
Topic#:
(212) 568-0365
Abraham Grossman
CBD 98-301
Title:RNA Detector-Molecule Methodology for Microorganism Biodetectors
Abstract:Current geopolitical strategies require effective warfare countermeasures to protect U.S. forces against biological and chemical threats. Today's molecular biology and immunological detection technologies cannot be used to produce automated biodetectors useable in the battlefield. New, improved detection technologies are needed to minimize the impact of chemical and biological weapons on Army personnel and provide a means to construct automated biodetectors that can identify a very few particles of any hazardous agent, regardless of interferant background. The final objective of the work proposed is to develop a highly sensitive RNA probe methodology to identify pathogenic bacteria and viruses. The methodology will underlay the foundation of RNA based tests that will be easy to perform as a simple enzymatic reaction at 37 degrees C under isothermal conditions in a single test tube format and can identify less than 100 target molecules in a specimen. Because of its extreme simplicity, the test could ultimately be incorporated into a small, portable, personal deviceuseable in battlefield environments without special training. This technology goes beyond normal evolutionary development approaches because it breaks the existing paradigms of the diagnostic industry by demonstrating the technical feasibility of detecting a non-nucleic acid target using a nucleic acid detector system.

TACAN CORP.
2330 Faraday Ave
Carlsbad, CA 92008
Phone:
PI:
Topic#:
(760) 438-1010
Jeffrey Ives
CBD 98-301
Title:A Compact, Automated Unit For Identifying Microorganisms by Their Nucleic Acids
Abstract:Due to the serious hazards of biological warfare agents, advanced detection capabilities must be developed to protect personnel in a variety of field situations. Aerosols, in particular, are a likely means of delivering the potential biohazards. Analyzing these aerosols rapidly and accurately is critical to successful defense, and can involve several levels of physical and biochemical analysis. A key biochemical component of bacteria and viruses is their nucleic acid composition because multiple copies of a toxin or viral agent will be produced from the genetic information. Unfortunately, current methods and instrumentation to detect specific nucleic acid sequences are too slow, labor intensive, and require multiple instruments for processing and analysis. This Phase I proposal describes an innovative RNA methodology to isolate the target microorganisms, prepare the ribosomal RNA, and detect it with fluorescently labeled oligonucleotide probes. The method is designed to be completed in 30 minutes or less, and can be integrated into a compact, automated instrument suitable for demanding field applications. Sample handling will be entirely enclosed to ensure safety and simplify operation. The fundamental position of nucleic acids in many biomedical and biotechnological fields supports the commercial potential of an effective nucleic acid identifier.