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18 Phase I Selections from the 01.1 Solicitation

(In Topic Number Order)
INTELLIGENT OPTICAL SYSTEMS, INC.
2520 W. 237th Street
Torrance, CA 90505
Phone:
PI:
Topic#:
(310) 530-7130
Dr. Srivatsa Venkatasubbarao
CBD 01-100      Selected for Award
Title:Biomapper for Gene Extraction
Abstract:Intelligent Optical Systems (IOS), in collaboration with Tetracore LLC (Rockville, MD), proposes to design, construct, and demonstrate a novel, portable, self-contained, imaging microarray reader (referred to here as Biomapper) to rapidly identify the exposure of Army personnel to biological warfare agents, chemical agents, physical stress, infectious diseases, and other medical conditions that would impact their physical performance. IOS's Biomapper will be based on a novel imaging technique that will monitor biochemical-binding events on the surface of a microarray. The monitored area will be approximately 25mm in diameter and have a spatial resolution of about 10 microns, resulting in the fully parallel, continuous readout of about 100,000 sensor fields. In Phase I, IOS will demonstrate a proof-of-concept reader using human b-actin messenger RNA as the model target. In Phase II of this project the optical, electronic, and fluidic components will be integrated to create a portable unit for field use. There are many applications for a portable microarray unit. The proposed reader will find applications in the military as a diagnostic tool and as a prognostic tool. Clinical laboratories and emergency medical personnel are also likely to benefit from this reader. The reader could also be used as a diagnostic instrument, leading to more rapid treatment and a reduction in medical costs. This device will also be used in the pharmaceutical industry, biotech industries, and research laboratories.

LYNNTECH, INC.
7610 Eastmark Drive
College Station, TX 77840
Phone:
PI:
Topic#:
(979) 693-0017
Dr. Dalibor Hodko
CBD 01-100      Selected for Award
Title:New DNA/RNA Sequencer for Rapid Assessment of Exposure to Infectious Agents
Abstract:The importance of DNA/RNA-based assays for detection of diseases, genome sequencing, forensics, and environmental control is continually increasing. High cost and complexity of current instrumentation for DNA/RNA sequencing prohibits their use in the field or at the point-of-care. Increasing knowledge in gene functions, gene expression and pharmacogenetics calls for new methods for rapid assessment of diseases. The challenge is the development of low cost and portable method for sequencing of DNA and/or RNA molecules extracted from blood or urine samples, to recognize a specific sequence which points to eventual exposure to toxic or infectious agents. All conventional techniques are based on DNA/RNA molecule immobilization to a specific capture probe anchored to the surface using hybridization to a complementary DNA sequence of interest. Micro-array based probes require complex pre-synthesis and preparation of numerous capture probes that are often prone to non-specific binding. The proposed method is based on electrophoretically separating and directing a single DNA/RNA molecule fragment of interest through a simple, self-assembled nano-pore detector. Sample preparation and detection are performed on-chip using microfluidic principles, which provide instrument miniaturization. The method implies unprecedented sensitivity for each nucleobase as it passes through the detector and will offer a unique direct "reading" of a single DNA or RNA molecule with high speeds.The proposed micro/nanofluidic system for single molecule DNA and/or RNA sequencing will find a large number of applications in genomics and proteomics research, disease discovery and prevention, as well as in early detection of exposure to toxic and infectious agents at the point-of-care. Potential commercial applications of the developed method will range from pharmaceutical drug analysis to field monitoring of pathogens in biological warfare detection, food industry and environmental monitoring of microorganisms.

CHESAPEAKE PERL, INC.
387 Technology Drive
College Park, MD 20783
Phone:
PI:
Topic#:
(301) 405-0207
Ms. Terry Chase
CBD 01-101      Awarded: 14MAY01
Title:Mass Customization Biomanufacturing Process
Abstract:This proposal develops the basis for a self-tuning expression system exploiting fluorescing insect larvae. Chesapeake PERL, Inc., co-founded by Drs. Bentley and Pham, was formed to exploit insects as host organisms, where the eventual biomanufacturing process is comprised of parallel self-regulating "mini" larval bioreactors. The process is amenable to any production scale, and, importantly, is self-regulating based on readily monitored larval fluorescence. To be specific, Chesapeake PERL is developing a method of "mass customization" for recombinant protein manufacturing in insect larvae that will reduce costs by two orders of magnitude. "Mass customization" refers to a system where one process can make several different customized products and vary the scale of production from one to many units. The key to mass customization is that the process remains constant irrespective of product and scale. Chesapeake PERL envisions an automated assembly line of millions of larvae all producing recombinant protein and automatically harvested at the optimal point. Fluorescent marker proteins, exclusively licensed to Chesapeake PERL, enable this automation and on-line monitoring. When insect larvae are infected with a recombinant baculovirus expressing a product and fluorescent proteins, the fluorescence is evident through the larval skin. The fluorescence tracks the product yield and illuminates the critical window for optimal harvest. Then, automated machines facilitate harvest. The most fluorescent larvaes can have 500% more protein than the average. With this system Chesapeake PERL can produce recombinant proteins of superior quality and biological activity.Chesapeake PERL is developing a method of "mass customization" for recombinant protein manufacturing in insect larvae. With unprecedented automation, Chesapeake PERL's new manufacturing process is easily customized to produce virtually any recombinant protein, automatically, at high yield and low cost, with superior quality and biological activity. By lowering the cost of recombinant protein manufacturing by two orders of magnitude, Chesapeake PERL will enter markets where the use of recombinant proteins has been cost prohibitive.

ENVIROGEN, INC.
4100 Quakerbridge Road
Lawrenceville, NJ 08648
Phone:
PI:
Topic#:
(609) 936-9300
Dr. Robert J. Steffan
CBD 01-101      Awarded: 14MAY01
Title:Integrated Expression and Process Monitoring System for Biomanufacturing
Abstract:This Phase I Small Business Innovative Research project will involve the construction of DNA expression vectors that will facilitate the production and secretion of high levels of peptides and other fermentation products in bacteria. The vectors will allow for simultaneous production and secretion of the target peptide and a reporter peptide. A hallmark of the proposed project is the development of an on-line, real-time, fermentation monitoring system that will allow improved process control during the fermentation process. The proposed research will address the need for improved large-scale production of a wide range of peptides, including antibodies for detection and defense of chemical and biological weapons, and industrial enzymes for biocatalysis. The feasibility of this approach will be demonstrated by constructing the expression vectors and producing a commercially important lipase enzyme that can be used for creating enantio-pure feedstocks for the production of pharmaceutical and fine chemicals. The products of this Phase I research will be a series of versatile cassette-based expression vectors and host organisms designed for optimum expression and secretion of peptides, and a design for an on-line monitoring and process control system for industrial-scale fermentation. Both the vectors and fermentation control systems developed during this project will have a wide range of government and private sector applications. The technology will be useful for large-scale production of antibodies for detecting and inactivating chemical and biological warfare agents, and it will be useful for producing commercial quantities of a wide range industrial enzymes and non-proteinacious fermentation products.

COHERENT TECHNOLOGIES, INC.
655 Aspen Ridge Drive
Lafayette, CO 80026
Phone:
PI:
Topic#:
(303) 379-3132
Dr. Iain McKinnie
CBD 01-102      Awarded: 14MAY01
Title:Compact, Efficient, Rapidly-Tunable LWIR Transmitter
Abstract:Standoff detection, identification and tracking of chemical and biological warfare agents is critical to provide hazard warning and dewarning for forces and civilians, and to minimize disruption in deployment of forces. Differential Absorption Lidar (DIAL) can be used for remote sensing of chemical agents via their spectral signatures at MWIR and LWIR wavelengths. However, current gas-laser DIAL systems are not highly robust and cannot access certain critical wavelengths. All-solid-state LWIR laser systems using optical parametric oscillators (OPOs) are potentially more suitable for field deployment, but have so far been limited by poor electrical-optical efficiencies and by limitations of OPO materials such as AgGaSe2. A further requirement for rapid-tuning and variable bandwidth is also incompatible with conventional mechanically-tuned, fixed bandwidth OPOs. CTI proposes a robust, compact and efficient all-solid-state LWIR and MWIR laser source. The system implements efficient one-micron pump technology, enhanced conversion to MWIR, and efficient LWIR generation. A novel non-mechanical scheme provides rapid LWIR wavelength and bandwidth selection. Phase I will demonstrate the spectral control concept in an efficient LWIR OPO. Phase II will deliver a compact and rugged LWIR OPO. The program leverages CTI's experience in development and productization of MWIR and LWIR OPOs, and in flight qualified solid-state laser systems.Frequency-agile laser transmitters in the MWIR and LWIR are needed for commercial DIAL sensors for industrial chemical detection, pollution monitoring and leak detection. These lasers are also useful for scientific applications such as high resolution spectroscopy. With minor modifications, SWIR output can be generated as a diagnostic source for WDM communications. High efficiency infrared lasers are also attractive for wind-sensing, free-space communications, search and rescue beacons, IR countermeasures and medicine.

SOUTH BAY SCIENCE & TECHNOLOGY CO
7525 W. 1st St.
Playa del Rey, CA 90293
Phone:
PI:
Topic#:
(310) 647-0966
Dr. David B. Cohn
CBD 01-102      Awarded: 03MAY01
Title:Optimized Optical Parametric Oscillator (OPO) Converter for Solid State Standoff CB Sensors
Abstract:The objective of the proposed program is to develop and demonstrate a robust and efficient optical parametric oscillator for converting the output of a solid state, 1.06 micron laser to the 8-12 mmicron band for application to laser standoff chemical sensors. The converter will be based on nonlinear crystals that have much higher damage threshold than the conventional AgGaSe2 type and efficiency will be improved by placing these crystals in novel intracavity and extracavity resonator configurations. In order to improve lifetime, high damage threshold, multi-band, optics will be developed for the OPO resonator, involving both advanced optical polishing techniques and enhanced coatings. A prototype converter will be tested, resulting in a performance data base that will be used to develop preliminary transmitter designs applicable to low and high power sensor systems. Provides a critical component for advanced laser-based sensors for remote chemical detection and rangefinding with commercial applications in pollution monitoring, waste site remediation, and civil defense.

PHYSICAL SCIENCES, INC.
20 New England Business Center
Andover, MA 01810
Phone:
PI:
Topic#:
(978) 689-0003
Dr. John D. Lennhoff
CBD 01-103      Awarded: 11MAY01
Title:Adhesively Bonded Electrospun Membranes for Protective Clothing
Abstract:Physical Sciences, Inc. (PSI) has developed a novel method for the patterned deposition of a fiberized adhesive. This fiberized adhesive will be used to laminate conventional fabrics and electrospun chemical/biological warfare (CBW) agent barrier membranes, with minimal effect on fabric breathability. The fiberized adhesive deposition will be optimized for adhesive strength to the electrospun barrier membrane designed with a failure mode below the tear strength of the protective membrane. The wide range of adhesives applicable to the proposed method will permit selection of an adhesive with a simple cure mechanism, mechanical properties matched to the garment, and vapor permeability. During the proposed Phase I effort, PSI will optimize the fabrication of a laminated protective clothing structure for drape, strength, permeability to moisture, lauderability, and adhesive cure. We will optimize the balance between cohesive and adhesive bond failure to minimize electrospun membrane tearing during use. The Phase I effort will develop a new adhesive delivery technology and measure properties of the fabrics produced using it. The Phase II effort will design and build a continuous pilot scale line to fabricate the laminated fabric structure.The PSI patterned fiberized adhesive application method may provide an extra degree of freedom for adhesive deposition and therefore find widespread use in select adhesive laminated products. Textile applications where breathability and abrasion resistance are important will benefit most by the PSI method. The recreation apparel market has a consistent need for the type of multi-layer, breathable textiles that the PSI method may provide.

CHEMMOTIF, INC.
60 Thoreau Street, Suite 211
Concord, MA 01742
Phone:
PI:
Topic#:
(781) 376-9911
Dr. Amy E. Stevens Miller
CBD 01-104      Awarded: 01JUN01
Title:Colorimetric Sensors for End-of-Service-Life Indicators for Mask Filters
Abstract:ChemMotif has developed several new colorimetric vapor sensors. These will be tested as end-of-service-life indicators for carbon-filter gas-masks in Chemical Biological Defense applications. Modification of the ChemMotif sensors will be done to demonstrate their potential to meet the ESLI requirements. These sensors will permit safer and more cost-effective use of gas-mask carbon filters.Low-cost End-of-Life Service Indicators for chemical protective gear will provide assurance to chemical emergency response teams, including firefighters, law enforcement personnel, industrial workers, and environmental remediation personnel that their protective clothing is in working order on removal from storage and during use. This technology may also be applicable to organic vappor detection in industrial and environmental applications such as waste site assessment.

K&M ENVIRONMENTAL, INC.
2421 Bowland Parkway, Suite 102
Virginia Beach, VA 23454
Phone:
PI:
Topic#:
(757) 431-2260
Dr. Kirollos S. Kirollos
CBD 01-104      Selected for Award
Title:Colorimetric End-of-Service Life Indicator for Mask Filters
Abstract:The service member currently has no visual means to determine the residual service life of his or her respirator. Early pioneers recognized the acute need for an ESLI in the 1920's and later several authors addressed different ESLI construction concepts and designs. However, due to the instability of the selected colorimetric chemistries and/or the suspected poor mechanical properties of the support media, the devices were rendered impractical for ESLI use. The overall objective of this proposal is to develop non-specific, colorimetric ESLI chemistry concepts designed to target major classes of organic vapors and/or acid vapors, and to determine their feasibility through testing with chemical agent simulants. To achieve these objectives, the feasibility of producing two non-specific colorimetric chemistries will be pursued. The first approach involves the modification and adaptation of an existing proprietary technology owned by the proposing company. The second approach involves a novel non-specific colorimetric detection mechanism. A proprietary technology will also be utilized to overcome the drawbacks of the previous art. The ELSIs will be tested ex-situ against agent simulants in environmental chambers capable of controlled temperature, humidity and velocity conditions.The anticipated benefit of the proposed ESLI for respirator cartridges and/or canisters would be that it enables the user to visually monitor, in real time, the service life of the respirator filter in a quantitative and qualitative manner. The use of these products will result in reduced risk to the user of potential exposure to hazardous industrial contaminants. Non-specific colorimetric chemistries for acid vapors, warfare agents and organic vapors are considered the major components for the development of end of service life indicators. The high market demand of these products will be driven by the cost savings and the superior features of these products.

COMPACT MEMBRANE SYSTEMS, INC.
325 Water Street
Wilmington, DE 19804
Phone:
PI:
Topic#:
(302) 999-7996
Dr. Nina Lamba
CBD 01-105      Awarded: 15JUN01
Title:Super-Efficient, Low Toxicity, Dendrimer-Quaternary Ammonium Compound Biocides
Abstract:The threat of terrorist or military attack using biological or chemical warfare agents is one of the largest concerns to U.S. military forces. Development of defense initiatives that can be implemented to decontaminate personnel, equipment and buildings are required to counter the effects of such an attack. A series of dendrimer-quaternary ammonium compound (QAC) biocides have been identified that demonstrate potent antimicrobial activity against bacteria. These materials are more than 100 times more effective at killing E.coli than a comparable amount of free QAC. It is anticipated that these materials will be able to decontaminate chemical agents also. The goal of the proposed work is to develop these biocides for military defense applications. Materials will be synthesized, characterized and tested for antimicrobial activity. Optionally, we will explore the efficiency of these materials against chemical agents. We expect these materials to be effective against warfare agents, but non-destructive to equipment, electronics etc. These materials can be applied as a powder, in solution, or immobilized onto polymers, enhancing utility in pre-event installation measures as well as post-event decontamination. Successful development of these materials would provide a broad spectrum agent for the neutralization and decontamination of biological and chemical warfare agents. These agents could be used both to protect personnel and installations in the event of exposure, and as decontamination agents during clean-up. These materials are not expected to cause severe damage to materials of construction of buildings, equipment etc. The ability to use these materials in a number of forms will allow their use as air filters, solutions, aerosols, garments, ointments, water purification etc. More broadly, compounds and materials displaying biocidal activity will have commercial applicability to private sectors where prevention of biofilms is desired, including healthcare,. Polymers containing these biocidal agents will have utility wherever prevention of bacterial adhesion and colonization is required, e.g. protective clothing, food preparation areas, medical devices, air filtration systems, waterlines, etc.

LYNNTECH, INC.
7610 Eastmark Drive
College Station, TX 77840
Phone:
PI:
Topic#:
(979) 693-0017
Dr. Anuncia Gonzalez-Martin
CBD 01-300      Awarded: 26APR01
Title:An Array-Based Sensor for the Detection of Toxic Industrial Materials
Abstract:There is a critical need for the detection and identification of toxic industrial materials (TIM). This proposal concerns the development of an array-based chemical sensor that has a real time response, is highly sensitive and inexpensive, and requires a minimal attendance and maintenance. The array-based sensor will be composed of incrementally diverse conducting polymers. They are formed by a new lithographic fabrication method, and have unique advantages and detection capabilities compared to existing gas sensors. By controlling the properties of the individual elements of the array, a unique fingerprint will identify chemical agents. The fabrication procedure will allow the sensor elements to respond to the presence of TIMs in hazardous concentrations from naturally occurring backgrounds. The sensor will incorporate integrated circuits to control sampling and signal detection and analysis, including pattern recognition. During the Phase I project, the feasibility of the monitor design at the bench scale will be demonstrated in the detection, identification, and quantification of TIMs. During the Phase II project, a prototype sensor will be fabricated, field tested, and delivered to the Government. The prototype will be design to be integrated in the joint chemical agent detector (JCAD). There are large potential commercial applications involving the use of an array-based chemical sensor to: (i) to detect the presence of toxic and regulated chemicals in industries as well as in commercial and residential applications,; (ii) to analyze complex mixtures found in the medical, food, beverage, agronomic and perfume industries, such as human breath, perfumes, beers, foods, mixtures of solvents, pesticides, etc.; (iii) to monitor the effectiveness of remediation efforts; (iv) to sense pesticides in water and in fresh produce, to assess livestock waste, and to monitor airborne pollen levels, etc.

TRITON SYSTEMS, INC.
200 TURNPIKE ROAD
Chelmsford, MA 01824
Phone:
PI:
Topic#:
(978) 250-4200
Dr. Erik Handy
CBD 01-300      Awarded: 12APR01
Title:New Generation of Saw Sensors Coatings for the Detection of Toxic Industrial Materials
Abstract:Triton Systems Inc. proposes to develop a new generation of SAW sensor coatings for the detection of TIMs. The proposed innovation employs a convenient, highly-controlled, and reproducible method to deposit conductive and nonconductive substrate coatings on SAW sensors. Since the proposed coatings can be used to operate on different transduction modes, they exhibit a high level of chemical Independence leading to enhanced selectivity and sensitivity. In Phase I, both conducting and non-conducting coatings will be deposited on SAW crystals and their response to TIMs will be characterized. Systematic changes in coating properties will be examined in order to optimize their response characteristics. Since these coatings have high chemical flexibility, introduction of specific chemical functionalities to further increase their chemical diversity will also be attempted. In phase II, the most promising coatings identified in phase I will be used to construct and demonstrate a TIM sensor. We will also examine the viability of using these conductive coatings in chemiresistor sensors.The proposed family of coatings will expand the collection of chemically independent interfaces available for SAW sensors. When incorporated in electronic noses, the proposed SAW sensors will find commercial applications in the food, beverage, and cosmetic industries. These sensor are also well suited to environmental pollution monitoring and industrial process monitoring.

EIC LABORATORIES, INC.
111 Downey Street
Norwood, MA 02062
Phone:
PI:
Topic#:
(781) 769-9450
Dr. Michael D. Gilbert
CBD 01-301      Awarded: 18APR01
Title:Barrier Coatings for NBC Mask Rubber Materials
Abstract:Spray applied acrylic coatings are proposed as barrier materials for the Air Force MCU-2/P and MBU-19/P gas masks, to provide protection against a broad range of chemical warfare agent (CWA) threats. The MCU-2/P and MBU-19/P are fabricated from silicone and EPDM rubber, respectively. They afford exceptional comfort, durability and fit. However, these materials offer only limited protection against chemical threats. The acrylic-based coatings will address this shortcoming by providing continuous thin film barrier coverage of the rubbers. The coatings are formed by spraying of mixtures of commercially available monomers and then curing using ultraviolet initiators. The coating process will be both rapid and inexpensive. The versatility of the acrylic polymerization process will allow the coatings to be specifically tailored to optimize compatibility with the substrate rubbers, while maintaining high barrier properties. An investigation will be conducted to assess the viability of acrylate coatings as barriers for silicon and EPDM rubbers. Samples will be directly compared to standard fluoroelastomer coated materials. Coating adhesion, rubbery modulus, mechanical robustness and strength will be measured. Tests will also be conducted to determine the resistance of these barrier coatings when exposed to a wide range of solvents, chemicals and chemical warfare agents.Many of the hoses used in automotive or industrial applications are made of rubbery materials. These are often used to carry aggressive chemicals or are exposed to harsh chemical environments, which attack or otherwise decompose the rubber. Easily applied, elastomeric coatings would do much to prolong the life of these materials and might eliminate the use of more expensive rubbers in some applications, resulting in substantial cost savings. Almost all rubbers are subject to oxidation and ozone attack, which causes the rubber to crack and fail over time. A mechanically robust barrier coating might prolong the life of rubbers used in such demanding applications as vibration or shock mounts.

METSS CORP.
720-G Lakeview Plaza Blvd.
Columbus, OH 43085
Phone:
PI:
Topic#:
(614) 842-6600
Dr. Donald Bigg
CBD 01-301      Awarded: 23APR01
Title:Improving Chemical Protective Capabilities of Silicon and Ethylene Propylene Diene Rubber (EPDM) Class Rubber Materials
Abstract:The Chemical and Biological Defense (CBD) Agency needs a coating for MCU-2/P and MBU-19/P facemasks that provides 24 hours of protection against such chemical warfare agents as GD, HD, and VX. Such a barrier material is expected also to provide protection against many toxic industrial materials. The MCU-2/P and MBU-19/P masks are made from silicone rubber EPDM rubber, respectively. Both rubbers are flexible, so the desired barrier coating must exhibit comparable flexibility. The coating also must adhere to the mask while it is subjected to flexing and stretching over a range of temperatures between -48 and 49 oC. METSS proposes to develop at least one polymer-based coating that will provide the desired level of performance and protection for each mask. The approach is to identify candidate polymers from the general classes of barrier polymers discussed in the proposal, and to experimentally verify the performance of one or more barrier polymers or polymer composites that meet(s) the requirements set by the CBD Agency. METSS plans to use its extensive knowledge in CWA resistant materials to select and examine the performance of pure polymer coatings as well as polymers whose barrier performance has been enhanced by the addition of exfoliated nano-scale platelets.The benefits of improving the protective capabilities of the rubbers currently used in making facemasks will be considerable. Chemical warfare is insidious and deadly. All efforts to protect soldiers and civilians in targeted areas will result in the saving of lives and reduction in long-term, detrimental health effects. In addition, there are numerous commercial and industrial applications related to working with toxic chemicals where improved protective equipment can increase worker safety and health. Emergency response personnel also will benefit from improved personal protective equipment. The chemically resistant barrier materials developed in this program may even find use in the growing area of biological protective equipment. METSS has identified a potential commercial partner and a manufacturer of facemasks to assist in the commercialization of innovative coatings that are developed in this SBIR program

PVD PRODUCTS, INC.
231 Andover Street
Wilmington, MA 01887
Phone:
PI:
Topic#:
(978) 694-9455
Dr. James Greer
CBD 01-303      Awarded: 23MAY01
Title:Improved Methods for the Polymer Coating of Surface Acoustic Wave (SAW) Crystals
Abstract:The need for high quality performance thin polymer coatings on surface acoustic wave (SAW) crystals has stimulated significant efforts in the areas of deposition and sensing technology. The surface uniformity and reproducibility of the coating process plays a significant role in the performance of the coated SAW components. Recently, a new matrix assisted polymer laser evaporation (MAPLE) technique has been developed for advanced polymer deposition. An opportunity is presented to improve chemical agent detection performance of advanced chemoselective polymer coatings through a more uniform, cost-efficient, and reproducible process. Specifically, the novel photothermal MAPLE process will be developed for SAW substrate coatings. The effect of MAPLE polymer deposition process performance of the SAW sensor will be examined for vapor sensitivity and device characteristics. The Phase I will include substantial materials characterization for uniformity and repeatability of the selected polymer films. A prototype design for automated deposition of the polymer coating will be presented. An optimized coating process for SAW devices will be implemented in Phase II in addition to an instrument prototype for polymer coating. Side-by-side comparison with aerosol spray coated SAW sensors will be included. Probability for Phase III commercialization of both the MAPLE process and instrumentation is considered high.The nation may expect to benefit from the success of the proposed R&D of a low cost, reproducible, uniform polymer coating process for quartz crystal SAW devices. The use of MAPLE processes and instrumentation will provide for a strong US manufacturing base of coating equipment.

SENTOR TECHNOLOGIES, INC.
9467 Manorwood Dr.
Mechanicsville, VA 23116
Phone:
PI:
Topic#:
(804) 550-0157
Dr. Natalia Levit
CBD 01-303      Awarded: 19APR01
Title:Development of an Automated Precision Coating System for SAW-Based Chemical Sensors
Abstract:This proposal describes a new automated precision polymer coating technology based on supercritical fluid processing. Highly uniform and reproducible polymer films and coatings are produced using a simple spray-on method known as Rapid Expansion of Supercritical Solutions or (RESS). The deposition process is automated through the use of a rotary stage and mechanical shutter and the entire process is controlled from a computer workstation. In phase I, polymer films will be applied to Surface Acoustic Wave (SAW) crystals and the film properties and sensor performance will be characterized to establish the feasibility of using the technique for the mass production of SAW-based chemical sensors. In phase II, a prototype automated precision coating system will be developed and commercialized.New polymer coating technologies are needed for a variety of applications. Potential commercial applications of chemical sensors developed using this coating technology include environmental characterization, military surveillance, medical diagnostics and industrial pollution monitoring. Additional commerical applications of this technology include advanced optical coatings, telecommunications and corrosion protection.

IDAHO TECHNOLOGY, INC.
390 Wakara Way
Salt Lake City, UT 84108
Phone:
PI:
Topic#:
(801) 736-6354
Dr. Deepika de Silva
CBD 01-304      Awarded: 10MAY01
Title:Freeze-dried reagents for real-time PCR
Abstract:Development of single use, freeze-dried PCR reagents for use with the Idaho Technology Ruggedized Advanced Pathogen Identification Device (RAPIDT system) or other commercially available real-time PCR devices is proposed. These reagents will have extended stability at ambient temperature thus allowing sensitive PCR methods to be used in non-laboratory environments for the rapid detection of biological agents. Preliminary results using freeze-dried reagents containing all the components required for a PCR reaction, including enzyme, oligonucleotide primers and fluorescently labeled probes have demonstrated the potential of the proposed system. These prototype reagents have already been successfully deployed with the RAPID in select USAir Force exercises. We propose to extend our work on the freeze-drying technology to maximize long-term reagent stability, maintain maximum fluorescent signal and implement protocols for the production of these reagents as a commercial product. It is anticipated that these reagents can be launched in military and non-military markets for use with a variety of commercially available real-time PCR instrumentation.As PCR is validated and deployed for the detection of infectious and biowarfare agents, a substantial market for ready-to-use reagents will be created. Idaho Technology can provide lyophilized, GMP compliant PCR reagents to support this need.

NANOSCALE MATERIALS, INC.
1500 Hayes Drive
Manhattan, KS 66502
Phone:
PI:
Topic#:
(785) 537-0179
Dr. Olga B. Koper
CBD 01-400      Awarded: 02MAY01
Title:Nanoparticle Based Hand-Portable Waterless Decontamination System
Abstract:The objective of this Phase I proposal is to develop novel decontaminating adsorbents based on Reactive Nanoparticle Technology to address chemical and biological threats. Nantek's reactive nanoparticles have demonstrated excellent results as reactive decontaminants for of both chemical and biological agents. Building on this research and success, Nantek proposes development and extensive evaluation of non-toxic nanoparticle formulations to be used to decontaminate personnel, cargo and airspace. A hand-portable prototype will be developed, various nanoparticle metal oxides and propellents will be evaluated as surface decontaminants against mimics of chemical and biological warfare agents. Thus, at the completion of Phase I, technical feasibility for the nanoscale metal-oxide based hand-portable waterless decontamination system for personnel, surfaces and airspace will be demonstrated.Successful completion of this research will lead to the development of emergency decontamination technologies to address contaminated airspace, personnel and surfaces for military and industrial applications.