---------- DARPA ----------

24 Phase I Selections from the 11.2 Solicitation

(In Topic Number Order)
Advanced Cooling Technologies, Inc.
1046 New Holland Avenue
Lancaster, PA 17601
Phone:
PI:
Topic#:
(717) 295-6061
Michael Ellis
SB112-001      Awarded: 11/3/2011
Title:Vapor-Liquid Pumping through Integral Separation
Abstract:Advanced Cooling Technologies, Inc. (ACT) is proposing to develop a pump capable of moving a two-phase fluid against large pressure gradients. This pump will accept fluid at any quality and will maintain outlet pressure regardless of inlet quality variation. Pumping will be provided by conventional means with a prime mover dedicated to each phase. To allow for this, the phases will be passively separated before entering the pump. Using a passive separation technique will simplify the overall design as complicated seals and moving parts are eliminated. This simplification will increase pump reliability and reduce manufacturing costs. In addition, the separation stage can accumulate liquid and gas. This feature will provide a buffer between inlet quality changes and volumetric flow changes at each pumping component. This buffer allows the two-phase pump to respond gracefully to inlet quality changes. Phase I will concentrate on the development and demonstration of the two-phase thermal management system operating with the two-stage pump design. In Phase II, ACT intends to work with a commercial partner to develop a packaging strategy and design, fabricate, and test an initial two-phase pump prototype, which will be sized for a 20 kW thermal management system.

Influent Corporation
10201 Maple Leaf Court
Ashland, VA 23005
Phone:
PI:
Topic#:
(804) 550-3490
Tim Lucas
SB112-001      Awarded: 11/7/2011
Title:Pump for Vapor-Liquid Mixtures of Refrigerants
Abstract:Current cooling systems are limited by the heat carrying capacity of the fluid, which is in turn limited by the pumping technology to move the fluid. This proposal allows a much higher heat capacity in the fluid, due to allowing the fluid to go from a 5% to 95% quality within the cooling circuit, which is enabled by an innovative and unique pumping technology that will be developed in this proposal. With this wide span of qualities in the fluid you can create an active pumping heat pipe system, with associated higher heat capacities. This represents a major breakthrough in thermal management of systems of this type.

Mainstream Engineering Corporation
200 Yellow Place Pines Industrial Center
Rockledge, FL 32955
Phone:
PI:
Topic#:
(321) 631-3550
Jerald Wagner
SB112-001      Awarded: 11/1/2011
Title:Vapor-Liquid pump for Mixed Phase Refrigerant
Abstract:Current refrigerant pumping technology lacks the ability to circulate a liquid-vapor mixture with substantial flow resistance, which impedes development and implementation of two- phase cooling technology. Common pump designs will hydro-lock and mechanically fail instantly or suffer performance loss and eventually fail due to cavitation or worn components. Mainstream’s solution is based on an existing miniature compressor designed for vapor- compression cycles. This compressor can be modified to be a pump with capabilities of maintaining thermal management system pressures in a refrigerant system where fluid quality can vary between 5% and 95%. The Phase I effort will focus on physical testing to experimentally optimize Mainstream’s miniature pump to operate with two-phase refrigerant. With this data, a Phase II scale pump will be designed. Phase II will further develop a pump capable of 1000 hours of continuous operation between maintenance intervals. In summary, the proposed solution can meet or exceed all of the requirements set forth in the solicitation and has a high probability of success due to Mainstream’s previous expertise with robust design of advancing technology.

TIAX LLC
35 Hartwell Avenue
Lexington, MA 02421
Phone:
PI:
Topic#:
(781) 879-1269
John Dieckmann
SB112-001      Awarded: 10/28/2011
Title:Scroll Pump for Vapor – Liquid Mixtures of Refrigerants
Abstract:The purpose of this topic is to develop a pump that can pump two-phase, liquid and vapor mixtures of common refrigerants and heat transfer fluids, without oil lubrication. The challenges of pumping in this regime are well known: • Two-phase, liquid-vapor refrigerant mixtures are a mixture of saturated liquid and saturated vapor, by definition meaning that there is zero net positive suction head. Many pump types are highly susceptible to cavitation under these conditions, leading to rough operation, poor performance, and erosion and ultimate destruction of pump elements, especially centrifugal pump impellers. • The common fluorocarbon refrigerants and heat transfer fluids are generally low in liquid viscosity and offer no boundary lubrication type lubricity, making mechanical design of a pump to handle these fluids challenging with respect to both mechanical friction losses and the wear life of rotating shafts, bearings, and pump elements. To meet the need for a pump that can handle two-phase, saturated liquid-vapor mixtures of refrigerants and similar heat transfer fluids, TIAX proposes to develop a scroll type pump that is tailored specifically for this purpose.

Digital Solid State Propulsion LLC
6150 Sunrise Meadows Loop
Reno, NV 89519
Phone:
PI:
Topic#:
(775) 851-4443
Wayne Sawka
SB112-002      Awarded: 11/16/2011
Title:Direct Plasma Production from Electric Solid Propellant for High Velocity Launch Systems
Abstract:As an alternative to double-base propellants we propose electric solid propellants (ESP) as a safe, green, higher performance alternative to double-base propellants for high velocity electro thermal chemical (ETC) canons. These new energetic materials are inherently safe, with ignition only possible via the sustained supply of electrical power and not by spark, flame or even bullet impact. A major disadvantage of conventional double-base propellants for ETC use is their extremely low loading densities @ ~0.3g/cm3, required for plasma spreading within the combustion chamber (shell). Unlike those double-base propellants, ESPs can be packaged as a single monolithic grain igniting by one or more inert electrodes and allow 4-5 times greater loading densities. This reduction of parasitic volume of the ETC rounds will allow more ammunition to be transported by the Warfighter in less space.

Streamline Automation, LLC
3100 Fresh Way SW
Huntsville, AL 35805
Phone:
PI:
Topic#:
(256) 713-1220
Stelu Deaconu
SB112-002      Awarded: 11/7/2011
Title:High Velocity Insensitive Launch Systems
Abstract:Streamline Automation, LLC., is proposing a development program designed to explore the feasibility of achieving hyperkinetic launch capability with a novel electro-thermal gun concept. The new launch system will be insensitive materials compliant, will employ a highly efficient trigger technology which will simplify system design and operation and enable energy conversion efficiencies two to three times greater than those of chemically powered guns. During Phase I SA will demonstrate concept feasibility and features with a small caliber experimental system. In Phase II SA will perform analysis, optimization, component integration and testing of a larger caliber system and will develop a realistic plan for technology maturation through Phase III.

Systima Technologies, Inc.
1832 180th St. SE
Bothell, WA 98012
Phone:
PI:
Topic#:
(425) 487-4020
Jerry Berg
SB112-002      Awarded: 11/2/2011
Title:High Velocity Insensitive Launch Systems
Abstract:Systima is proposing to develop an end-to-end integrated solution to provide a high velocity, Long Range Ram Accelerator Mobile Field Howitzer (LRRAM-FH) system that will dramatically increase velocity and range capabilities over conventional artillery. The ram accelerator launcher will accelerate the projectiles to velocities greater than 2 km/s, which will extend the capability of modern kinetic weapons and kinetic energy levels. LRRAM-FH design will maximize the mission effectiveness by providing a long range strike capability within existing artillery units while not adversely increasing logistics requirements. The LRRAM-FH would integrate existing infrastructure for training/elevating the tube and fire control systems. The LRRAM-FH will have adequate mobility to be integrated within existing remote firebases. The ram accelerator is a hypervelocity launcher that has been demonstrated with muzzle velocity up to 2.7 km/s. A new configuration offers significant performance gains over conventional ram accelerators and improved field-ability. Use of kinetic energy rather than high explosives will help these high velocity projectiles be insensitive material (IM) compliant. This in turn improves the system’s performance in the field and provides increased value to the warfighter. Systima will develop the concept and evaluate integration/application methods/designs to optimize the performance during the Phase I program.

UTRON, inc.
9441 Innovation Drive
Manassas, VA 20110
Phone:
PI:
Topic#:
(703) 369-5552
David Kruczynski
SB112-002      Awarded: 10/31/2011
Title:Hydrogen Based High Velocity Insensitive Launch Systems
Abstract:The objective of this proposal is to further explore and refine the use of hydrogen based propellants for use in high velocity gun systems. This proposal will build on the ongoing and very novel research being conducted with direct combustion of hydrogen-oxygen mixtures at high initial pressures. While the research performed to date has demonstrated the performance potential inherent in this approach, there remains need for additional work on non-invasive ignition, propellant storage and production, and combustion kinetics. In addition, the potential of side injector use for further performance enhancement and/or acceleration reduction will be explored.

E-Line Ventures, LLC
363 7th Avenue, Floor 20
New York, NY 10001
Phone:
PI:
Topic#:
(347) 247-8607
Mr. Alan Gershenfeld
SB112-003      Awarded: 12/1/2011
Title:Online Graphic Novel/Sequential Art Authoring Tools for Therapeutic Storytelling
Abstract:Addressing PTSD and related stress reactions by Service Members and Veterans is one of our country’s most important public health challenges. Art therapy has been recognized as a powerful tool enabling “trauma survivors to symbolically express, process and contain feelings they find difficult or impossible to put in to words” (Buk, 2009). Enabling Service Members and Veterans to tell their stories – in a self-guided manner and/or therapist- assisted modalities could be effective in the treatment of PTSD and related stress reactions. The proposed program, designed by a team of leading comic creators and educators, digital media tool providers and military and psychological experts will feature four fully integrated components: Comic Authoring Tools: Easy-to-use comic authoring tools that can accommodate a range of abilities, styles and content; Educational Supports: Educational supports to help Service Members and Veterans improve their ability to express themselves through the comics; Psychological Health Supports: On-line resource center and psychological health and related supports to help ensure the safety and efficacy of the program; Curated Examples: A suite of comics by Service Members and Veterans and well- known comic creators that address combat-related PTSD and related stress reactions.

IDEAS
64 East Concord Street
Orlando, FL 32801
Phone:
PI:
Topic#:
(407) 601-7878
Mr. Richard Stone
SB112-003      Awarded: 11/17/2011
Title:Online Graphic Novel/Sequential Art Authoring Tools for Therapeutic Storytelling
Abstract:The overall objective of this Phase I proposal is to develop a template and plan for a web- based software with a simple, user friendly interface that assists in both storytelling and graphical content creation, thereby allowing individuals to relate their experiences directly or metaphorically, and then assess the efficacy of this tool to address the following questions. 1.What kinds and styles of images are most appealing to users? 2.What kinds of narrative tools are most useful? 3.What would be the most useful output methods for sharing the story with friends, colleagues, or therapists? 4.How do the benefits of this graphic novel tool differ from the already researched and identified value of art therapy in general? 5.Can such a tool be an attractive option for self-exploration for a person suffering with PTSD? 6.What kinds of online support and tools are required to facilitate the effectiveness of the tool? 7.Would creating a safe, social network for sharing be a valuable adjunct?

Kinection
4448 Morse Ct.
Napa, CA 94558
Phone:
PI:
Topic#:
(831) 359-8778
Mr. Douglas Nelson
SB112-003      Awarded: 11/17/2011
Title:The Warrior Stories Platform
Abstract:Arts and Narrative therapies have been shown to be beneficial for Veterans and Service Members traumatized by life experiences. The Warrior Stories Platform provides therapists and Service Members a tool to create rich, healing stories in a graphic novel format. The platform uses active assistance to provide authors support during the creation process by assisting them in structuring their story, finding appropriate art, and adding contextual sounds. Art libraries are provided in a variety of themes, and a rich symbol library helps authors express complex feelings without having to render concrete scenes in detail. Characters, backgrounds, props, and symbols are all highly configurable using proprietary technology, and all are searchable not just by name but also by emotion, mission type, event, and location. The platform allows use in individual, group, and therapist-supervised settings.

MYMIC LLC
1040 University Boulevard Suite 100
Portsmouth, VA 23703
Phone:
PI:
Topic#:
(757) 391-9200
Mr. Phillip Jones
SB112-003      Awarded: 11/4/2011
Title:Online Graphic Novel/Sequential Art Authoring Tools for Therapeutic Storytelling
Abstract:MYMIC LLC will team with Eastern Virginia Medical School (EVMS) and Psychic Bunny Inc. to develop the MyART system, a web-based, therapeutic system for supporting those with Post-Traumatic Stress Disorder (PTSD) and other emotional issues and their care team through the use of graphic art. MyART will be a highly individualized system that emphasizes simple and intuitive interfaces that support the individual user, regardless of: that person’s role in PTSD therapy, their involvement in active therapy, how PTSD is presenting, or their graphic narrative authoring skills. MyART will meet the user where that user is. MyART will enable the user to author professional quality graphical narratives that provide therapeutic benefit. It will include access to guides to assist these creation and therapy processes. It will include progressive art content and advanced management systems to aide access to that content. MyART will include the means to interact with other users as is appropriate to individual therapy. MyART will include an inherent assessment subsystem that will employ a variety of explicit and tacit processes to assess the systems effectiveness and performance in supporting each individual user and of the whole of the system. MyART will be developed with careful considerations to personal privacy and medical ethics.

Scientific Systems Company, Inc
500 West Cummings Park - Ste 3000
Woburn, MA 01801
Phone:
PI:
Topic#:
(781) 933-5355
Mr. Michael McComas
SB112-003      Awarded: 11/2/2011
Title:Sequential Art Authoring and Layout Toolkit for Therapeutic Storytelling (SALTS)
Abstract:SSCI will develop a toolkit for assisting people with limited previous writing experience to write and illustrate a graphic novel as part of a program of treatment for Post Traumatic Stress Disorder. This project will include built-in therapeutic aids for Art Therapy, Therapeutic Storytelling, and Narrative Therapy. In Phase I, we will analyze requirements and develop a detailed project plan for Phase II, including user testing and evaluation.

Smart Information Flow Technologies, d/b/a SIFT
211 N 1st St. Suite 300
Minneapolis, MN 55401
Phone:
PI:
Topic#:
(612) 789-0559
Mr. Dan Thomsen
SB112-003      Awarded: 10/24/2011
Title:SAGA: Sequential Art via Game Assistance
Abstract:The main challenges of treating post-combatant PTSD include getting patients into treatment, keeping them engaged, and motivating them to complete out-of-session take-home work. SAGA (Sequential Art via Game Assistance) addresses these challenges by providing PTSD patients an engaging and educational game environment that helps them understand and communicate their trauma while creating a graphic novel. SAGA employs easy-to-use tools to help patients create visually compelling stories, disassemble, and understand how stories work. Sample stories from prior patient’s testimonials on completing therapy provide additional critical education and motivation to patients. By collecting metrics on patients’ progress and emotional triggers, SAGA can schedule exercises to fit individual patients’ therapeutic needs. SAGA includes the most successful memes from popular games like World of Warcraft, Rock Band and Farmville to increase patient engagement in treatment, and allow PTSD patients to support each other. SAGA ensures that every successful step a patient makes in therapy can encourage other patients to do the same.

Total Immersion Software, Inc.
1 Enterprise Pkwy Suite 330
Hampton, VA 23666
Phone:
PI:
Topic#:
(703) 415-7577
Dr. Amy Kruse
SB112-003      Awarded: 11/10/2011
Title:Online Graphic Novel/Sequential Art Authoring Tools for Therapeutic Storytelling
Abstract:IN-Comics begins with a research and development effort by Total Immersion Software, Inc. to create an innovative web-based personal computer platform for soldier self-expression as a new tool for therapeutic storytelling. The resulting technology that will emerge blends the principles of art therapy with innovative online graphic novel and sequential 2D and 3D art authoring tools and online delivery systems to create a new form of self-expression, social media and community support. This effort creates a unified strategy from high concept to deployment, merging art therapy theories and research with practical experience of veteran therapeutic practitioners, and folds that insight into an authoring and delivery platform created by expert graphic tool designers and engineers who have successfully fielded innovations in military server delivery infrastructure. Guiding this vision is the inspiration of storytelling mass-media experts and graphic novel professionals and veteran software and program managers, with the resulting system and its content ultimately coupled with expert- verified metrics in the back end and with ongoing usability and effectiveness reviews. IN- Comics are designed to create a new form of digital expression for therapy, education, training and instruction in both government and civilian sectors of our society.

Carley Technologies, Inc.
1924 Glen Mitchell Road
Sewickley, PA 15143
Phone:
PI:
Topic#:
(412) 953-8818
Eric Malloy
SB112-004      Awarded: 11/15/2011
Title:GENNET: Advanced System for Synthesis of High Fidelity Social Networks to support SHIELD
Abstract:Our objective is to provide a semi-automated system to generate and verify scalable, high fidelity, multi-dimensional, social networks with realistic distributional, temporal and spatial characteristics. This will enable the user to generate networks varying in size, node attributes, geo-temporal characteristics, consistency with other networks, and reflection of known subpopulations, in formats used by standard network analytic packages. Generative algorithms will be informed by empirical data and theoretical findings about network generation. We build synthetic networks using a multi-algorithm approach employing subpopulation identification, empirical seeding, constraint satisfaction, and multi-network alignment. Synthesized networks are compared against real networks varying in size, media usage, and activities conducted by network members. Veridicality is assessed on multiple dimensions; network density, distribution of centrality measures, temporal characteristics, spatial characteristics, and evolutionary properties. Deltas between temporal slices will be validated for key actors and topologies at both the subpopulation level and overall network level. Each aspect will be compared to corresponding measurements in known networks at the node, group, motif, and topology levels. A secondary feature of the proposed system will be the ability to layer onto the synthetic network hidden covert networks or purposive networks designed to accomplish some task.

Charles River Analytics Inc.
625 Mount Auburn Street
Cambridge, MA 02138
Phone:
PI:
Topic#:
(617) 491-3474
Corey Lofdahl
SB112-004      Awarded: 11/9/2011
Title:A Tool for Agent-Based System Produced Emergent Networks (ASPEN)
Abstract:Social network analysis is a powerful method for making sense of an increasingly interconnected world. Developing social network analysis tools requires datasets with which to test them, but sharing datasets is problematic because of privacy issues. Initial attempts to anonymize network datasets proved ineffective due to de-anonymization attacks that leveraged publically available information to identify individuals. Synthetic datasets provide an alternative, but it is difficult to ensure that a synthetic network is sufficiently similar to a real network for tool development. To address this issue, our system for Agent-Based System Produced Emergent Networks (ASPEN) imbues agents with social and behavioral theories, has them interact in a simulation, and generates a synthetic network from the simulation log. The simulation network is then compared to a real network using network metrics to assess their similarity or fidelity. These metrics are then fed to an evolutionary algorithm (EA) that varies the behavioral inputs to the agent-based simulation to improve the synthetic network’s fidelity. Synthetic networks thus emerge from the behavioral interactions among agents rather than being directly specified.

Intelligent Automation, Inc.
15400 Calhoun Drive Suite 400
Rockville, MD 20855
Phone:
PI:
Topic#:
(301) 294-5267
Yalin Sagduyu
SB112-004      Awarded: 11/1/2011
Title:High Fidelity Synthesis of Dynamic Social Networks using Measurement-based Calibration
Abstract:We propose to develop a high fidelity approach of synthesizing measurement-calibrated dynamic social networks. Social network research needs access to realistic graph datasets for testing social network theory and developing network algorithms. However, the available social network data are either typically small or cannot be readily shared due to privacy issues. To address these challenges, a unifying robust framework is proposed to incorporate novel techniques for high fidelity synthesis of social network data with dynamic graph measurement calibration. Online social network (OSN) data will be leveraged to form social interaction graphs. This approach provides the flexibility to capture link formations dynamically as functions of interaction intensities by adding temporal dynamics. We propose to apply measurement-based calibration of diverse graph models. Different network analytical functions (including user attributes, interaction, subgraph populations and temporal variations) can be represented by statistical time-varying descriptions in these graph models. Network synthesis results will be thoroughly evaluated in terms of similarity with OSNs by using diverse fidelity criteria such as statistical metrics, application-level benchmarks and geometric/topological properties. The accuracy-complexity trade-offs in multi-dimensional fidelity objectives will be balanced by introducing a closed-loop control mechanism that can automatically update model selection and optimize parameters in graph fitting.

Perceptronics Solutions, Inc.
3527 Beverly Glen Ter
Sherman Oaks, CA 91423
Phone:
PI:
Topic#:
(818) 788-4830
Amos Freedy
SB112-004      Awarded: 10/24/2011
Title:SHIELD (Novel Techniques for the Synthesis of High Fidelity Social Network Data)
Abstract:This proposal is for developing Novel Techniques for the Synthesis of High Fidelity Social Network Data (SHIELD). The objective is to develop scalable methods, tools and techniques to synthesize and validate high fidelity social network data and to provide these to researchers in a modular, easy-to-use system. Our innovative program will integrate a set of social science tools for working with social networks; these include respondent driven sampling, consensus analysis, influence models, exponential random graph models, and the economy of relations. Using these tools, we will develop new scalable methods, tools and techniques to synthesize and validate high fidelity social networks which are similar enough to real networks such that applications developed with their data will work on corresponding real data. The essential features of these modeled networks are: they are longitudinal (i.e., they will account for change in nodes and links across time); they are dynamic (i.e., they will account for regular flows across relational interactions); their members respond to their dynamics; they involve multiple types of links, each with multiple attributes; and they involve multiple types of connected entities each with multiple attributes.

Agave BioSystems, Inc.
P.O. Box 100
Ithaca, NY 14850
Phone:
PI:
Topic#:
(607) 272-0002
Dr. Joel Tabb
SB112-005      Awarded: 11/2/2011
Title:Ionic Liquid-Based Dried Biological Specimen Materials
Abstract:Dried blood spots (DBS) analysis has many advantages over standard liquid blood sample testing including: small sample size, ease of blood sampling, room temperature storage, minimal storage size requirement, and ease of shipping. Although these advantages are significant, there are still problems associated with the current DBS systems. The drying of blood spots on the DBS cards can be uneven, leading to uneven distribution of biomolecules throughout the blood spot. This problem can be confounded by the fact that samples can undergo chromatography during DBS application. The paper-based DBS cards can also react with various components of the blood samples, causing oxidation and/or inactivation of antibodies, enzymes, cytokines and other important analytes. In this Phase I, Agave BioSystems proposes to develop novel blood storage media based on ionic liquids (ILs). These novel ionic liquid-based materials will have improved biocompatibility and flow characteristics, which will be major advantages over current filter paper-based DBS cards.

Ceres Nanosciences, LLLP
10900 University Blvd.
Manassas, VA 20110
Phone:
PI:
Topic#:
(800) 615-0418
Mr. Ben Lepene
SB112-005      Awarded: 11/10/2011
Title:Nanotrap Enabled Dried Biofluid Collection Paper
Abstract:We have developed a novel nanotechnology that overcomes major roadblocks that limit the ability to collect, process, analyze and store biological samples at ambient temperatures over extended periods of time. Ceres will fabricate and demonstrate Nanotrap-enabled dried biofluid sample collection paper technology suitable for a range of biofluids (whole blood, plasma, saliva, urine, food matrices) and analyte classes (proteins, peptides, metabolites, toxins, nucleic acids). The proposed technology is specifically designed for scientific research, monitoring of health and exposure and evaluation of countermeasure efficacy where standard sample collection, preservation techniques and equipment are impractical, inefficient and unreliable. The results of this Phase I effort will extend our patented Nanotrap sample preparation technology for sample collection applications for use in DoD point-of- need sample collection as well as routine clinical and personalized medicine applications.

Connecticut Analytical Corporation
696 Amity Road Route 63
Bethany, CT 06524
Phone:
PI:
Topic#:
(203) 393-9666
Mr. Joseph Bango
SB112-005      Awarded: 10/24/2011
Title:Improved Dried Biological Specimen Materials, Recovery and Processing for Diagnostics
Abstract:Dried blood spot (DBS) analysis has gained recognition in recent years in basic science and clinical diagnostics. This impetus relies upon its numerous advantages over the collection, transport and storage of blood samples obtained by conventional venipuncture. Although there is wide-spread interest in DBS, optimization of sample collection and extraction methods, as well as, the subsequent analyses by standard analytical methods is still in its infancy. Therefore, preliminary experiments comparing different extraction methods (e.g. alcohol incubation and electrospray transfer) were carried out and analyzed by enzyme linked immunosorbent assay (ELISA). We propose that electrospray ionization-transfer offers superior extraction to that of the standard chemical methods by 1) efficient extraction of analytes from the DBS substrate and 2) concentration of the analyte.

Luna Innovations Incorporated
1 Riverside Circle Suite 400
Roanoke, VA 24016
Phone:
PI:
Topic#:
(434) 220-2509
Ms. Blaine Butler
SB112-005      Awarded: 11/14/2011
Title:Encapsulation Matrix for Improved Biological Specimen Storage and Recovery
Abstract:Luna Innovations will establish the feasibility of modifying our novel bio-encapsulation techniques for enhanced preservation of biological samples. The proposed technology has been successfully applied to protein and whole cell encapsulation, providing significant improvements in storage life-time and temperature stability. This unique preservation method has the ability to reduce analyte loss while still maintaining analyte integrity. During Phase I, Luna will develop modified encapsulation matrices for the preservation of two different biological samples (blood and urine). In order to demonstrate enhanced recovery of analytes, various biomarkers (DNA, RNA, and cytokines) will be quantified as a function of preservation time, temperature, and degree of dehydration. Phase II will focus on increasing the storage lifetime of the samples as well as extending the range of suitable storage environments. Concurrently, Luna will be working to adapt this novel bio-encapsulation technique for integration into applications such as therapeutic drug monitoring, newborn screening, biomarker discovery and qualification research.

Spectrasilk, Inc.
196 Boston Avenue Suite 1700
Medford, MA 02155
Phone:
PI:
Topic#:
(339) 221-3038
Dr. Eun Gil
SB112-005      Awarded: 11/9/2011
Title:Multifunctional Stabilization Matrix for Biological Samples
Abstract:The need for improved biological sample collection and processing for diagnostic applications has grown with the advent of new assays based on small sample sizes and high performance analytics. In contrast to the advances in these analytics, the ability to collect, stabilize, ship and utilize such samples has lagged in terms of development. We offer a relatively simple platform based on recent discoveries of the unique features of silk proteins as remarkably robust stabilization matrices for biological samples. Preliminary data demonstrates remarkable collection and stabilization of labile substrates, such as enzymes, antibodies, vaccines and hemoglobin in the silk matrix, with retention of function over months. This feature derives from the unique protein block copolymer structure of silk, dominated by hydrophobicity with small nanodomains for limited entrained water. This novelty extends well beyond the stabilization features, as the protein is relatively simple to utilize in an aqueous or lyophilized format, can be cast into any form or shape, provides stability of entrapped biologicals over months without refrigeration, can be formed into credit-card formats with optical or Electromagnetic (e.g. RF) features to permit interrogation of the activity of the contents, or can be deconstructed to remove the contents for analysis.