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18 Phase I Selections from the 12.2 Solicitation

(In Topic Number Order)
Extend Biosciences Inc.
48 Russell St. #3
Cambridge, MA 02140
Phone:
PI:
Topic#:
(732) 599-8581
Laura M. Hales
CBD12-101      Awarded: 3/25/2013
Title:Enhancing the Pharmacokinetic Profile of Protein-based Drugs
Abstract:Many protein-based drugs have limited efficacy due to a short half-life or require intravenous delivery because of low bioavailability when given subcutaneously. Extend Biosciences is developing proprietary carrier molecules that will allow proteins to access a transport pathway for efficient delivery to the vascular space and then maintain a sustained presence in circulation. This would be of particular importance in the development of longer-lasting versions of bioscavenger proteins that could then be delivered subcutaneously and become bioavailable within minutes of administration. In this project, one of Extend Biosciences’ carrier molecules will be conjugated to two bioscavenger proteins of interest to the military as further proof-of-concept for the technology. The modified bioscavengers will be assayed in vitro to ensure that the carrier molecule does not disrupt functional activity. Following success in Phase I, the Phase II studies would test the modified bioscavengers for their improved half-life and bioavailability when delivered subcutaneously in an appropriate animal model, and test whether the modified protein induces toxicity or an immune response. This project will demonstrate the feasibility of improving the half-life and bioavailability of bioscavenger proteins that could be applied to numerous other protein-based drugs including those used in Chemical and Biological Defense treatments.

CFD Research Corporation
215 Wynn Dr., 5th Floor
Huntsville, AL 35805
Phone:
PI:
Topic#:
(256) 327-5294
Hongjun Song
CBD12-102      Awarded: 4/9/2013
Title:An automated, high throughput, resin-free device for large scale protein purification
Abstract:Protein manufacturing is of paramount importance to chemical and biological defense applications. High-throughput protein purification is a critical need for rapidly scaling the target protein product. Existing purification methods primarly rely on synthetic-resin based chromatography, which is time-consuming, labor-intensive, expensive, and consequently, ill- suited for developing rapid countermeasures to chemical/biological threat. To overcome these limitations, we propose to develop and demonstrate a novel high-throughput, resin-free technology for automated protein recovery and purification. Our technology based on novel multi-scale electrokinetics for protein purification enables order-of-magnitude improvement in investment and operating cost as well as human labor. In Phase I, we will design, fabricate, characterize and demonstrate a pilot-scale prototype to establish proof-of-principle of the proposed technology. The designs of the purification device will be optimized using high-fidelity simulation tools. The fabricated device will be tested and demonstrated in our well-equipped bioengineering laboratories. In Phase II, a full-scale purification system with additional design refinements will be developed for enhanced performance and manufacturability. The purification module will be integrated with COTS technologies for automated operation. The Phase II prototype will be demonstrated for continuous, long-term purification of target proteins from composite sample matrix. The final product will be fully automated, high throughput and low cost system for rapid purification of desired proteins

Luna Innovations Incorporated
1 Riverside Circle Suite 400
Roanoke, VA 24016
Phone:
PI:
Topic#:
(434) 220-0159
Michael Danilich
CBD12-102      Awarded: 3/25/2013
Title:Affinity Matrix System for Large-Scale Protein Purification
Abstract:Though vast improvements have been made in various protein expression platforms, limitations arise in the implementation of current purification methods. Traditional chromatographic methods are time intensive, carry heavy material costs, and are difficult to scale - reducing the benefits achieved in protein expression. Affinity-binding methods are promising for scale-up and swiftness of purification, but the necessary resins can be expensive and difficult to adapt to purify proteins from new expression platforms. To respond to this need, Luna proposes the development of a low-cost affinity matrix with a high surface area to provide substantial binding regions for targeted proteins of interest. This system will allow for the purification of any protein expressed with the cellulose binding module and intein self-cleaving tag, an expression possible with many proteins in a wide variety of host organisms. The implementation of this low-cost affinity matrix and widely applicable purification system will allow for fast, simple, inexpensive purification of any target protein.

Celadon Laboratories, Inc.
6525 Belcrest Rd. Suite 521
Hyattsville, MD 20782
Phone:
PI:
Topic#:
(301) 683-2118
Raymond Peterson
CBD12-103      Awarded: 12/14/2012
Title:Software for Design and Validation of DNA Nanostructure Surface Patterning
Abstract:A continuing threat to U.S. military personnel and civilians is exposure to toxic chemicals and pathogens. While there exist methods and systems for vaccine development, agent detection, and assessment of exposure and immune response, it remains imperative to investigate next-generation technologies that have the potential to improve by an order of magnitude their cost, sensitivity, specificity, and speed. A promising technology is the self-assembly of charge-patterned DNA nanostructures on a flat surface. In order for patterned DNA nanostructures to become commercially viable, the technology needs investigation of the thermodynamics of self-assembly, both with and without charged pendants; development of predictive algorithms that take into account pendant thermodynamics and modified nucleic acids; and automated design software. Towards this end, Celadon will partner with Dr. Jason Kahn and Dr. David H. Mathews, both internationally recognized experts in nucleic acid chemistry and computation. During Phase I, the Celadon/Kahn/Mathews team will assemble origami surface with patterned hydrophilic and hydrophobic patches on ~5 nm scale; confirm with atomic force microscopy and fluorescence; conduct temperature-dependence studies and develop thermodynamic algorithms to predict assembly in presence of pendant groups; construct Phase II plan to develop predictive algorithm and software; and demonstrate prototype browser-based and Cloud-enabled user interface.

Parabon NanoLabs, Inc.
11260 Roger Bacon Drive Suite 406
Reston, VA 20190
Phone:
PI:
Topic#:
(703) 689-9689
Steven Armentrout
CBD12-103      Awarded: 12/6/2012
Title:Design Automation Software for Biomimetic Surface Presentation with DNA Origami
Abstract:A development program is proposed for a design automation software package that enables the generation of complex surfaces, with user defined or automated definition of point charge and hydrophobic/hydrophilic properties, using DNA origami as a presentation substrate. Building upon existing software for DNA origami design, this project will result in software able to optimize DNA staples and other origami modifications to achieve target charge and hydration force distributions. The objects to be generated with this software will be able to mimic any of the many surface/surface interactions found in biological or technological applications. Such design software will significantly expand the capabilities of users to produce systems that are capable of highly specific interactions with target macromolecular species.

EPIR Technologies Inc
590 Territorial Drive, Suite B
Bolingbrook, IL 60440
Phone:
PI:
Topic#:
(630) 771-0203
Silviu Velicu
CBD12-104      Awarded: 10/22/2012
Title:Detection of Liquid Contaminants on Surfaces Using Hyperspectral Imaging
Abstract:Quick detection of Chemical/Biological (CB) agents in the field can provide critical reconnaissance and contamination avoidance. CB agents pose a serious threat to both civilian and military sectors, and present techniques rely on dangerous collection methods, active measurement through external infrared (IR) sources, and/or are time-consuming. EPIR proposes to provide critically needed improvements through the development of a passive standoff hyperspectral long wavelength infrared (LWIR) focal plane array (FPA) with a polarimetric capability that will exploit cold sky reflectance, and spectroscopy techniques to identify CB agents quickly, accurately, and on the move. Phase I will focus on spectral measurements of a chemical stimulant, and system modeling and design based upon the measurements. Milestones for Phase I include the measurement of the hyper-spectral datacube for a stimulant, and system specifications given in terms of the possible contaminants. Phase II will focus on prototype system design, construction and characterization. Phase III will focus on field-deployable system manufacturing and packaging.

Physical Sciences Inc.
20 New England Business Center
Andover, MA 01810
Phone:
PI:
Topic#:
(978) 689-0003
Jay Giblin
CBD12-104      Awarded: 11/9/2012
Title:Advanced LWIR Hyperspectral System for On-the-Move Standoff Detection of Liquid Contaminants on Surfaces
Abstract:Physical Sciences Inc. (PSI) proposes to utilize an innovative LWIR hyperspectral technology coupled with advanced data processing algorithms for non-contact, passive standoff detection of liquid contaminants on surfaces. The technology will address the US Army’s need for fast, passive, on-the-move, non-contact detection of ground contamination during reconnaissance missions. The Phase I effort will focus on demonstrating the feasibility of the proposed on-the-move approach by validating signal models with field data collected against liquid agent simulant particles of relevant sizes. Improved SCR (Signal- to-clutter ratio) will be demonstrated through the use of innovative algorithms in conjunction with polarization-enhanced hyperspectral detection and an optional LWIR illumination sub- system for contrast enhancement. In a successful Phase II program, PSI will develop and evaluate a TRL 5 prototype system capable of on-the-move (~10 mph) detection of contaminant particles with surface density of 0.5 g/cm2 with Pd > 90%.

SA Photonics
130A Knowles Dr.
Los Gatos, CA 95032
Phone:
PI:
Topic#:
(415) 977-0553
Jim Coward
CBD12-104      Awarded: 11/7/2012
Title:Detection of Liquid Contaminants on Surfaces Using Hyperspectral Imaging
Abstract:SA Photonics is pleased to propose the development of the SENTRY hyperspectal situational awareness system for the detection of liquid chemical agents in the asymetrical terrorist warfare scenario. SA Photonics will be teaming with world renown experts from the University of Arizona for the development of the SENTRY system. Additionally, SA Photonics will be leveraging technology from numerous Navy, Air Force, DARPA, Army, and MDA programs to develop a Phase II prototype for demonstration to CBD customers.

Lynntech, Inc.
2501 Earl Rudder Freeway South
College Station, TX 77845
Phone:
PI:
Topic#:
(979) 764-2200
Alan Cisar
CBD12-105      Awarded: 2/22/2013
Title:Unpressurized High Density Oxygen Storage with Hydrogen Peroxide
Abstract:Closed circuit breathing apparatus (CCBA, rebreather systems) where exhaled CO2 is removed and con-sumed oxygen replaced, offer much longer working times on a single gas charge than open circuit ones (SCBA). This is achieved through the use of high pressure compressed oxygen. When CCBA units are used in conflict areas this requires the regular delivery of hazardous cargo (high pressure oxygen, 3,000 psi) into combat areas. Clearly a means of supplying oxygen without this hazard is desirable. This can be accomplished using a solution of hydrogen peroxide as the oxygen source. A liter concen-trated hydrogen peroxide at ambient pressure can be decomposed to produce the same amount of oxy-gen as contained in a one liter cylinder at high pressure. With the right catalyst the decomposition can be carried out at any temperature where the solution is liquid. Lynntech has developed oxygen supplies based on hydrogen peroxide decomposition that start immediately, can be throttled over the full range from off to full flow and can be repeatedly cycled for extended operating times. The ability to shut down oxygen production and restart it offers distinct advantages over other oxygen generators by letting the system supply oxygen the like a compressed gas cylinder.

Mainstream Engineering Corporation
200 Yellow Place Pines Industrial Center
Rockledge, FL 32955
Phone:
PI:
Topic#:
(321) 631-3550
Justin Hill
CBD12-105      Awarded: 12/31/2012
Title:Light-weight Low-volume High Oxygen Storage Density Material for Self-Contained Breathing Apparatus
Abstract:This proposal details the method of fabrication for a hierarchical hybrid nanomaterial for oxygen storage vessels. These vessels exceed the volumetric and gravimetric storage capacity of an oxygen cylinder by 330% and 215%, respectively. It is estimated that this oxygen storage device will weigh about 12% of a standard low pressure 2216 psi O2 cylinder and be only 4% of the total volume. The oxygen storage device does not depend on adsorption or a chemical reaction, which reduces the complexity of the system, making it easy to use. Mainstream will determine the optimal fill/seal/release mechanism to ensure close control over the release of oxygen to match the user’s needs. Furthermore, the novel storage medium comprises a safe technology that would gain immediate market penetration in the area of oxygen storage and delivery in the personal protection equipment, rescue equipment, aviation, and medical industries.

Busek Co. Inc.
11 Tech Circle
Natick, MA 01760
Phone:
PI:
Topic#:
(508) 655-5565
Yu-Hui Chiu
CBD12-106      Awarded: 2/1/2013
Title:High Capacity Supported Ionic Liquids for Carbon Dioxide Capture in Rebreather Systems
Abstract:Busek Co. Inc. and Ionic Research Technologies, LLC propose to develop a novel carbon dioxide and water removal technology using ionic liquids for closed circuit self-contained breathing apparatus that will extend its operating lifetime, while simultaneously reducing overall weight and logistic burden. Ionic liquids offer unique characteristics including high capacity CO2 capture, tunable gas selectivity, humidity capture, and negligible vapor pressures. The proposed technology is a significant advancement on current state-of-the- art technologies in use for CO2 scrubbing applications. The Phase I effort will demonstrate the feasibility by identifying suitable ionic liquid systems through advanced modeling predictions and laboratory validation tests. The phase I option will produce a sub-scale proof-of-concept CO2 scrubbing device utilizing supported ionic liquid technology. The results of Phase I will provide a baseline for the design of the Phase II prototype.

Lynntech, Inc.
2501 Earl Rudder Freeway South
College Station, TX 77845
Phone:
PI:
Topic#:
(979) 764-2200
David Battaglia
CBD12-106      Awarded: 1/29/2013
Title:Regenerable Carbon Dioxide Removal Technology Based on Novel Macroporous Ion Exchange Resins for Closed-Circuit Breathing Apparatus
Abstract:Currently, the U.S. Military encounters many scenarios where soldiers must enter hazardous areas, yet still maintain isolation from chemical and biological contaminants as well as hazardous materials. This requires the soldiers’ respiratory tract be isolated from encountering these contaminants. To accomplish this, the soldiers wear protective suits and/or masks which include a respiratory isolation system, more commonly called a rebreather. Current carbon dioxide scrubbers, which absorb exhaled carbon dioxide within the rebreather, are inherently prone to dusting, caustic, generate heat, and have single use lifetimes, causing risks to the soldier and increased logistical costs associated with ice packs and shelf life. Lynntech proposes a safe and novel solution to address these concerns by developing a scrubber based off a high surface area macroporous ionic resin with advanced grafting techniques using amines to create a fully regenerable sorbent to be used for carbon dioxide capture within the self contained breathing apparatus. This sorbent eliminates dusting, operates at a much more neutral pH value, has increased carbon dioxide capacity, and is more tolerant of high levels of humidity. Additionally, the sorbent eliminates ice packs by creating no noticeable heat of reaction and thus reduces the overall costs of the rebreather apparatus.

Paragon Space Development Corporation
3481 E. Michigan Street
Tucson, AZ 85714
Phone:
PI:
Topic#:
(520) 382-4817
Sebastian Padilla
CBD12-106      Awarded: 1/11/2013
Title:Regenerable, heat-Abating, humidity-Neutralizing, Carbon diOxide Removal System (RANCOR)
Abstract:Paragon Space Development Corporation (Paragon) will demonstrate the feasibility and benefits of our Regenerable, heat-Abating, humidity-Neutralizing, Carbon diOxide Removal System (RANCOR) for providing carbon dioxide and humidity recycling and removal for Self-Contained Breathing Apparatus (SCBA). RANCOR utilizes space-age humidity control technology combined with tailored CO2 and H2O absorbing materials to provide a fully reusable and regenerable humidity and carbon dioxide (CO2) removal solution. RANCOR provides humidity control that substantial reduces the amount of water (H2O) vapor that must be removed therefore reducing exothermic heat production from humidity removal. The scrubbing elements are fully regenerable though a simple service procedure that induces complete regeneration, ensuring repeatable multi-use capacity. No disposable consumables are required. The Phase I effort will focus on analysis and laboratory testing to validating the RANCOR approach and reduce risk by quantifying the benefits of the RANCOR elements and testing to ensure long term reusability. Phase I and Phase I Option lay a solid foundation for prototype development in Phase II and commercial application. The RANCOR system is fully reusable, reduces SCBA cooling requirements, and offers the potential for significant improvements in logistics and cost of operation over existing systems.

CFD Research Corporation
215 Wynn Dr., 5th Floor
Huntsville, AL 35805
Phone:
PI:
Topic#:
(256) 327-0678
Yi Wang
CBD12-107      Awarded: 3/21/2013
Title:A Novel, Aerodynamics-augmented Continuous Ionization System for Electrostatic Collection of Bioaerosols
Abstract:Safe and efficient air ionization and filtration technology compatible to biodefense applications is of paramount importance. Current ionization methods are energy-intensive, costly, prone to ozone generation, or inefficient, and consequently ill-suited for building protection applications. To overcome these issues, we propose to develop and demonstrate a novel bioaerosol ionization and collection system for autonomous, round-the-clock air filtration and protection. The proposed device harnesses advanced aerodynamics-augmented ionizer design and electrode material optimization to enable significant improvement in throughput, ionization efficiency, ozone generation, and maintenance requirements and operating cost. In Phase I, we will perform a comparative study of the most promising design using high-fidelity simulation tools, followed by prototype fabrication and experimental characterization to demonstrate a laboratory-scale prototype and establish proof-of-principle of the proposed technology. In Phase II, novel designs for electrostatic collection of the charged aerosols will be explored and tested, which will be combined with the finalized ionizer design to form an integrated, automated air filtration system. The integrated systems will be refined for enhanced performance (ionization and removal efficiencies and ozone generation, energy consumption, and operating cost), ease of operation, and manufacturability. It will be extensively tested using composite biological sample matrix at various environmental and loading conditions. The final product will be a fully automated, continuous, safe air ionization and collection system with ready deployability in real environments.

Milow Ltd.
174 East Bay Street
Charleston, SC 29401
Phone:
PI:
Topic#:
(843) 727-6526
Gideon Rosenberg
CBD12-107      Awarded: 2/26/2013
Title:Continuous Ionization System for Electrostatic Collection of Bioaerosols in Building Protection Applications
Abstract:This proposal presents a method and technology for continuous ionization of bio-aerosols and airborne particles in building protection applications to enable effective removal of the ionized particles from the air flow by an electrostatic collection device. The proposed ionization method is based on a concept of combining a novel method of direct induction charging by an electric field with diversion of the air streamlines into many small passageways where field shapers are used to amplify the field intensity. No replenishing of consumables is needed for this process, it does not generate ozone and the efficient ionization in its low pressure-drop passageways consumes less power than HEPA filtration for building HVAC systems. The main objective of the proposed effort will be to demonstrate the ionization performance of a prototype that will be designed, built and tested in autonomous and continuous operation mode during a period of one week, including air monitoring to detect any ozone levels over the normal. The prototype features and the test results conducted in Phase I will be summarized in a final report, including evaluation of the induction charging characteristics for various types and sizes of particles.

Nanohmics, Inc
6201 East Oltorf St. Suite 400
Austin, TX 78741
Phone:
PI:
Topic#:
(512) 389-9990
Mike Durrett
CBD12-108      Awarded: 4/11/2013
Title:Rapid Sample Transport in Austere Environments
Abstract:Attaining rapid transportation of samples 50-1000 miles in an austere environment requires air transportation. Past applications have included UAV’s which transport the sample to selected location and drop the sample via guided parachute These systems can be quite accurate but they are expensive and relatively large. Balloons are an attractive alternative. They are inexpensive, compact and can be quite efficient at transporting reasonable loads long distances. When coupled with an active altitude control system, wind prediction can be employed to guide the balloon to desired regions. Key to this operation is good knowledge of and dynamic forecasting of nearby wind structures. Nanohmics proposes to develop a sample balloon delivery system that incorporates room temperature stabilization of biological samples, GPS and 2 way satellite communications, active altitude control system, onboard computer control of communications, wind measurement and prediction and flight path optimization. Passive temperature control will be applied to the sample and instrumentation and direct integration with JPADS or other meteorological reporting and modeling applications. This system can be controlled remotely or flown autonomously. This effort will build on Nanohmics’s past experience in the development of the GlideLine parachutist navigation system.

Piasecki Aircraft Corporation
519 West Second Street P.O. Box 360
Essington, PA 19029
Phone:
PI:
Topic#:
(610) 521-5700
Brian Geiger
CBD12-108      Awarded: 4/19/2013
Title:Rapid Sample Transport in Austere Environments
Abstract:Piasecki Aircraft proposes to meet the challenge of delivering a biological or chemical sample maintained at less than -20 deg Celcius over a range of up to 1000 miles with a precision landing with a portable unmanned aerial system capable of a vertical takeoff, cruise flight range typical of a fixed wing platform, and able to perform a precision landing. The system consists of a lifting quadrotor that air-launches a fixed wing, packable UAV with threshold 450 mile range and a soft precision landing system using a parachute capable of 50 yard accuracy. An electric cooling system using a peltier junction maintains the sample temperature at -50°C throughout the flight. En-route the system can accept updated flight plan data, and provided health and status information via remote telemetry. The Phase I development work will produce a preliminary design including CAD models and an operator interface to directly support a follow-on proof of concept prototype demonstration in Phase II.

STARA Technologies, Inc.
61 S. William Dillard Drive
Gilbert, AZ 85233
Phone:
PI:
Topic#:
(480) 850-1555
Glen R. Bailey
CBD12-108      Awarded: 5/14/2013
Title:Rapid Sample Transport in Austere Environments
Abstract:In austere and inaccessible environments, transport of critical medical, chemical and/or biological samples can be difficult. In this SBIR Phase I, a technical feasibility trade space is used to evaluate technologies used to preserve biomolecules, such as DNA, RNA, and bacterial at room temperatures; transport samples from austere locations to safe recover areas, recover samples after precision delivery to any specified location; establish a transport command, control, and monitoring system. In the feasibility trade space, key technology components and parameters are analyzed and rated for how well they support meeting technical objectives. The output of the trade space is a feasibility rating for each concept evaluated. System components evaluated include processes, methods, and equipment used to collect biological samples; methods and equipment used to store biological samples; use of balloons to transport samples using wind motion from austere locations to safe areas for recovery; use of guided parafoils to deliver samples to specified locations with GPS accuracies; a near real-time command and control system with world- wide coverage using SATCOM. The sample storage method is simplified by storing at room temperatures. Transport is simplified by using custom latex balloon capable of lifting a variety of system weights to selectable altitudes.