|Acquisition Program: || Objective: ||Develop a technology and method for real-time measurement of nonhuman primate respiratory tidal volume that is suitable for use in a Class 3 biological safety cabinet. The end product must withstand decontamination with formaldehyde and hydrogen peroxide gases, be operable within a sealed safety cabinet, and provide real-time, streaming tidal volume measurements accessible by a computer located outside of the sealed safety cabinet.
|| Description: ||Aerosol exposures of nonhuman primates are routinely conducted in biodefense research studies to evaluate the efficacy of medical countermeasures developed to protect against the threat of biowarfare agents. Because these studies are conducted with high consequence pathogens, all aerosol exposures are conducted in Class 3 biological safety cabinets. Continuous measurement of the respiratory tidal volume of the animal is needed in order to accurately control and measure the desired challenge dose. A real-time tidal volume measure will allow challenges for individual animals to be shortened or extended based on individual respiratory performance and the required challenge dose. While technologies currently exist for measuring respiratory parameters of nonhuman primates, none is suitable for use in the closed environment of a biological safety cabinet, rugged enough to withstand repeated decontamination procedures, and dynamic enough to allow for real-time incorporation of respiratory measurements into the aerosol control process. A respiratory measurement technology and method meeting these requirements will enable more accurate aerosol challenge studies to be conducted, speed development of medical countermeasures, and reduce the costs of countermeasure development.
|| ||PHASE I: Develop system design and concept for real-time tidal volume output, suitability for formaldehyde gas and hydrogen peroxide decontamination, and feasibility in Class-3 biological safety cabinet.
|| ||PHASE II: Develop and assemble prototype. Demonstrate prototype functionality in a closed chamber or biological safety cabinet. Displaying real-time tidal volume values on a standard computer station while measurements are being taken. Repeat demonstration and measurement after decontamination with foramldehyde and hydrogen peroxide gases.
|| ||PHASE III: A system meeting these requirements could be used in a range of applications in both biodefense research and commercial preclinical research and development. Inhalation exposure systems are commonly used in pharaceutical and toxicological research applications. A robust real-time respiratory measurement system suitable for use in the environment described would have translational applications in each of these fields.
|| References: ||1. JM Hartings and CJ Roy, "The automated bioaerosol exposure system: Preclinical platform development and a respiratory dosimetry application with nonhuman primates," Journal of Pharmacological and Toxicological Methods, 49 (2004):39-55
2. TK Besch, DL Ruble, PH Gibbs, and ML Pitt, "Steady-state minute volume determination by body-only plethysmography in juvenile rehsus monkeys," Laboratory Animal Sciences, 46 (1996):539-544
|Keywords: ||respiratory dosimetry, aerosol challenge, nonhuman primate|